Grafting of Epidermolysis Bullosa Wounds Using Cultured Revertant Autologous Keratinocytes

Inclusion Criteria: * Clinical diagnosis of EB (simplex, junctional or dystrophic) * Areas of revertant skin that has been confirmed by biopsy * 18 years or older subject willing and able to give consent * Confirmation of EB diagnosis by immunofluorescence (IF), electron microscopy (EM), and genetic testing confirming mutation * At least 100 to 200 cm2 of open erosions on the trunk and/or extremities suitable for skin grafting * Able to undergo adequate anesthesia to allow grafting procedures to take place Exclusion Criteria: * Medical instability limiting ability to travel to Stanford University Medical Center * The presence of medical illness expected to complicate participation and/or compromise the safety of this technique * Active infection with HIV, hepatitis B, or hepatitis C * Active infection in the area that will undergo grafting * Evidence of a systemic infection * Current evidence or a history of skin cancer in the area that will undergo grafting * Active drug or alcohol addiction * Hypersensitivity to vancomycin or amikacin * Receipt of chemical or biological study product for the specific treatment ofEB in the past six months * Positive pregnancy test or breast-feeding