Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animals. The investigators aim at generating haploid stem cells from human germ cells that are a useful tool in genetic research and screening.
Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animal models. haploid cells are easier for genetic manipulations, such as knock-in and knock-out of genes, and therefore should make a better experimental model. The investigators aim at generating haploid stem cells from human haploid germ cells.
Study Type
OBSERVATIONAL
Enrollment
10
Collected germ cells will be cultured in medium. The germ cell cultures will be infected with reprograming factors. Rare colonies with characteristic stem cell morphologies will be expanded.
Rambam medical center
Haifa, Israel
NOT_YET_RECRUITINGWeizmann institute of science
Rehovot, Israel
RECRUITINGPatients donated samples
No samples collected to date
Time frame: June 2011-November 2013
No secomdary outcome
Time frame: June 2011 - November 2013
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.