Smoking Research Study

Inclusion Criteria: Common criteria to smokers and non-smokers 1. Subject is able to understand the information provided in the Subject Information sheet and Informed Consent Form (ICF) 2. Subject has signed the ICF 3. Subject of Caucasian origin 4. Aged from 23 to 55 years 5. Having acceptable health conditions as judged by the Investigator at the screening visit based on clinical laboratory parameters (clinical biochemistry, urine analysis and standard haematology), spirometry, serology, urine drug screen, vital signs, physical examination, ECG, and medical history Specific to smokers 6. Current smoker based on self-reporting who smoke 5 to 15 commercially available non-mentholated conventional cigarettes (no brand restrictions) with a maximum tar yield of 10 mg (ISO method, as labelled on the cigarette package) smoking at least for the last 3 consecutive years. The smoking status will be verified at screening based on a urinary cotinine test (cotinine ≥200ng/ml) 7. Subject is willing to smoke according to the smoking regimen of the study Specific to non-smokers 8. Non-smoker based on self-reporting who have not used any tobacco or nicotine containing product including commercially available cigarettes, hand-rolled cigarettes, cigars, pipes, snuff, electronic cigarettes, similar devices, and nicotine replacement therapy within the last 12 months. The non-smoking status of the subject will be verified at screening based on a urinary cotinine test (cotinine \<200ng/ml) Exclusion Criteria: Common criteria to smokers and non-smokers 1. As per judgement of the Investigator, any subject who cannot participate in the study for any reason (e.g., medical, psychiatric and/or social reason) 2. Any clinically relevant gastrointestinal, renal, hepatic, neurological, haematological, endocrine, oncological, urological, immunological and cardiovascular diseases or any other condition including clinically significant abnormal laboratory parameters that, in the opinion of the Investigator, could jeopardise the safety of the subject or impact the validity of the study results 3. Lung cancer, upper respiratory tract cancers, chronic respiratory diseases or any other clinically significant pulmonary diseases according to the judgement of the Investigator 4. Any medical conditions requiring smoking cessation (e.g., recent acute cardiovascular event, diabetes mellitus, Chronic Obstructive Pulmonary disease) 5. Subject with body mass index \< 18.5 or ≥ 30 kg/m2 6. As per judgement of the Investigator, medical conditions which require or will require in the course of the study a medical intervention (e.g., start of treatment, surgery, hospitalisation) which may interfere with the study participation and/or study results 7. Donation or receipt of whole blood or blood products within 3 months prior to the screening visit 8. Participation in any clinical studies within 3 months before the screening visit 9. Current or former employee of the tobacco industry, or of their first-degree relatives (parent, sibling, child) 10. Employee of the investigational site or any other parties involved in the study or of their first-degree relatives (parent, sibling, child) 11. Enrolled in the same study at a different time (i.e., each subject can be in the study population only once) 12. Subject who is legally incompetent, physically or mentality incapable of giving consent (e.g., emergency situation, under guardianship, subject in a social or sanitary establishment, prisoners or subject who are involuntarily incarcerated) 13. Positive alcohol test and/or history of alcohol abuse that could interfere with subject's participation in study 14. Positive urine drug tests (amphetamines, barbiturates, benzodiazepines, cannabinoids, cocaine and opiates) 15. Positive serology test (HbsAg, HCV, HIV) Specific to smokers 16. Subject who has used any nicotine-containing products other than commercially available cigarettes (either a tobacco-based product or nicotine-replacement therapy) as well as electronic cigarettes and similar devices within 1 month prior to the screening visit Additionally, women of childbearing potential must be excluded if: 17. Subject is pregnant (does not have negative pregnancy tests at screening and at admission) or breastfeeding 18. Subject does not agree to use an acceptable method of effective contraception: intrauterine device, intrauterine system, established use of oral/injectable/implantable/transdermal hormonal methods, barrier methods of contraception (condoms, occlusive caps) with spermicidal foam/gel/film/suppository, vasectomised partner or true abstinence (periodic abstinence and withdrawal are not effective methods) from screening until the end of the safety follow-up period