Metabolic Availability of Lysine From Rice

The Hospital for Sick Children5 enrolled

Overview

The purpose of this study is to investigate the digestibility of the amino acid lysine in cooked rice in adult men using stable isotope technique.

The investigators objective is to determine the metabolic availability (MA) of lysine in cooked white rice protein using the indicator amino acid oxidation (IAAO) technique. Five men will receive graded levels (20, 40, and 60, and 70%) of the lysine requirement of 35 mg/kg/d as a crystalline AA mixture,and cooked rice protein (20, 40 and 60%), respectively.

Study Type

INTERVENTIONAL

Allocation

Purpose

HEALTH_SERVICES_RESEARCH

Masking

NONE

Enrollment

Conditions

Healthy Adults

Interventions

Amino Acid Crystalline Lysine Amino Acid MixDIETARY_SUPPLEMENT

crystalline lysine from AA MIX (20, 40, and 60% of the mean lysine requirement of 35 mg/kg/d) Lysine will be given in the form of L-Lysine-HCl, Sigma-Aldrich Brand.

Cooked Rice containing LysineDIETARY_SUPPLEMENT

Lysine (20,40 and 60 % of the mean lysine requirement) from Cooked Rice

Eligibility

Sex: MALEMin age: 18 YearsMax age: 50 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy Adult males (18 to 50years old) Exclusion Criteria: * A history of recent weight loss or illness * Use of any medication at the time of entry into the study.

Locations (1)

The Hospital for Sick Children

Toronto, Ontario, Canada

Outcomes

Primary Outcomes

Metabolic Availability of lysine

3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

Time frame: Baseline

Metabolic Availability of lysine

3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

Time frame: 15 min after intake of the fourth hourly meal (before tracer infusion))

Metabolic Availability of lysine

3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

Time frame: 30 min after intake of the fourth hourly meal (before tracer infusion))

Metabolic Availability of lysine

3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

Time frame: 45 min after intake of the fourth hourly meal (before tracer infusion))

Secondary Outcomes

Metabolic Availability of Lysine

4 breath samples will be taken to provide F13CO2 measurement (Level of phenylalanine oxidation).Breath samples will be collected and analyzed for 13CO2 enrichment.

Time frame: Post Tracer Infusion (between 150 and 240 min of tracer infusion)

Data from ClinicalTrials.gov

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