Urinary Proteomics Analysis for Sepsis and Prognosis

Chinese PLA General Hospital60 enrolled

Overview

As a noninvasive examination, urinary proteomics is a very useful tool to identify renal disease. The purpose of the present study was to find differential proteins among patient with SIRS and sepsis(included survivors and non-survivors), and to screen potential biomarkers for the early diagnosis of sepsis and its prognosis. Urinary proteins were identified by iTRAQ labeling and LC-MS/MS. The bioinformatics analysis was performed with the Mascot software and the International Protein Index (IPI) and the Gene Ontology (GO) Database and KEGG pathway Database. The differentially expressed proteins were verified by Western blot by another sample collected from clinical.

Study Type

OBSERVATIONAL

Enrollment

Conditions

SIRS Sepsis

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Male and female aged 18 years old and over; * clinically confirmed infection; * fulfilled at least two criteria of systemic inflammatory response syndrome * (a) core temperature higher than 38 °C or lower than 36 °C * (b)respiratory rate above 20/min, or PCO2 below 32 mmHg * (c) pulse rate above 90/min, and * (d) white blood cell count greater than 12,000/μl or lower than \< 4,000/μl or less than 10% of bands. Exclusion Criteria: * younger than 18 years of age; * acquired immunodeficiency syndrome; * reduced polymorphonuclear granulocyte counts (\< 500 μL-1); * died within 24h after admission into the ICU, or refused to participate in the study, or declined treatment during the period of observation.

Urinary Proteomics Analysis for Sepsis and Prognosis

Overview

Conditions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Central Contacts