Effects of Calorie Restriction on Accumulation of Old, Modified Proteins in Abdominally Obese Adults

Mayo Clinic29 enrolled

Overview

This study is being done to understand the effects of calorie restriction and weight loss on muscle protein metabolism in adult men and women.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Insulin Resistance

Interventions

Calorie RestrictionBEHAVIORAL

Participants will work with the CRU dietetics staff to plan a diet that will result in their losing 1-2 pounds per week over 16 weeks. They will wear an accelerometer to track there activity during these weeks.

Control (normal living)BEHAVIORAL

Participants will continue their normal living for entire time of the study. They will be asked to wear an accelerometer for the next 16 weeks and check in weekly at the CRU to exchange accelerometers.

Eligibility

Sex: ALLMin age: 45 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy * BMI will be between 30 kg/m2 - 38 kg/m2 and waist circumference for women ≥ 88 cm and men ≥ 104 cm * Age 45 to 65 years. * Male and Female Exclusion Criteria: * Active coronary artery disease * BMI \< 30 kg/m2 Participation in structured exercise (\>2 times per week for 30 minutes or longer) * Smoking * Medications known to affect muscle metabolism (beta blockers) * Renal failure (serum creatinine \> 1.5mg/dl) * Chronic active liver disease (AST and ALT \> 3 times normal) * Fasting blood glucose \> 126 mg/dl * Anti-coagulant therapy (warfarin/heparin)

Locations (1)

Mayo Clinic

Rochester, Minnesota, United States

Outcomes

Primary Outcomes

Change in muscle protein synthesis from baseline to 16 weeks

The investigators will determine the rate of incorporation of stable isotope amino acid tracers in skeletal muscle protein on average resting muscle protein synthesis rate (% new muscle protein per hour) and will be performed at baseline and 16 weeks after caloric restriction or control diet.

Time frame: Measured at week 0 (baseline) and week 16

Secondary Outcomes

Change in insulin sensitivity from 0 (baseline) and 16 weeks

The investigators will determine insulin sensitivity at week 0 (baseline) and 16 weeks into caloric restriction or control diet. The measurement is made by hyperinsulimic euglycemic clamp and reported as μmol per kilogram of fat free mass of infused glucose needed to maintain glycemia.

Time frame: Measured at week 0 (baseline) and week 16

Data from ClinicalTrials.gov

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