Cytoplasmatic maturation is an important event in the general maturation of of the oocyte. Mitochondria is one of the major organelles involved in the process of cytoplasmatic maturation. The aim of this study is to investigate the presence and distribution of the mitochondria in the cytoplasma of immature oocytes that are retrieved in the routine in-vitro maturation cycle with intracytoplasmatic sperm injection (ICSI). These oocytes will not undergo ICSI because of their immaturity. Usually, they are discarded. In addition to these immature oocytes the investigators shall also investigate the mitochondria in mature oocytes that underwent ICSI but did not become fertilized and are discarded.
Study Type
OBSERVATIONAL
Enrollment
20
Fixation and staining by MitiTracker Green FM
Observation with Laser scanning confocal microscopy
Hillel Yaffe Medical Center
Hadera, Israel
The patterns of mitochondrial distribution within the cytoplasma of oocytes during nuclear maturation
Identification the different patterns of mitochondrial distribution in correlation with the 3 stages nuclear maturation in human oocytes
Time frame: Three months
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