Diabetes(both types) are recognized by high levels of glucagon in the circulation. Glucagon is known to increase blood glucose, and might therefore contribute to the respective diseases. Under some circumstances the gut hormone GLP-1 inhibits the glucagon secretion. The investigators aim to identify the impact of GLP-1 on the glucagon secretion, at increasing blood glucose levels in healthy subjects, in patients with type 2 diabetes, and in patients with type 1 diabetes. The investigators think that the effect of GLP-1 on the glucagon secretion might be dependent of blood glucose levels.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
30
Continuous iv. infusion of the native gut hormone GLP-1 (0.2 pmol/kg/min) for 210 minutes. Combined with a step wise glucose clamp using 20% w/w glucose iv. infusion reaching plasma glucose value of 15 mM starting at fasting plasma glucose (30 min per step)
Continuous iv. saline infusion (NaCl Isotonic) for 210 minutes. Combined with a step wise glucose clamp using 20% w/w glucose iv. infusion reaching plasma glucose value of 15 mM starting at fasting plasma glucose (30 min per step)
Continuous iv. infusion of the native gut hormone GLP-1 (0.4 pmol/kg/min) for 210 minutes. Combined with a step wise glucose clamp using 20% w/w glucose iv. infusion reaching plasma glucose value of 15 mM starting at fasting plasma glucose (30 min per step)
Continuous iv. infusion of the native gut hormone GLP-1 (0.8 pmol/kg/min) for 210 minutes. Combined with a step wise glucose clamp using 20% w/w glucose iv. infusion reaching plasma glucose value of 15 mM starting at fasting plasma glucose (30 min per step)
Gentofte University Hospital
Hellerup, Denmark
Plasma Glucagon response to the GLP-1 infusion / glucose clamp
The collection of blood samples will be done during the 210 min GLP-1 infusion / glucose clamp-session
Time frame: At 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, 180, 195, 210 min
Plasma GLP-1 response to the GLP-1 infusion / glucose clamp
The collection of blood samples will be done during the 210 min GLP-1 infusion / glucose clamp-session
Time frame: At 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, 180, 195, 210 min
Plasma Glucose response to the GLP-1 infusion / glucose clamp
The collection of blood samples will be done during the 210 min GLP-1 infusion / glucose clamp-session
Time frame: At 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, 180, 195, 210 min
Resting Metabolic Rate response to the GLP-1 infusion / glucose clamp
Assessed by indirect calorimetry during the GLP-1 infusion / glucose clamp-session
Time frame: at 0, 120, and 180 min
Hunger scores response to the GLP-1 infusion / glucose clamp
Assessed by VAS during the 210 min GLP-1 infusion / glucose clamp-session
Time frame: At 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, 180, 195, 210 min
plasma Insulin response to the GLP-1 infusion / glucose clamp
The collection of blood samples will be done during the 210 min GLP-1 infusion / glucose clamp-session
Time frame: At 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, 180, 195, 210 min
plasma GIP response to the GLP-1 infusion / glucose clamp
The collection of blood samples will be done during the 210 min GLP-1 infusion / glucose clamp-session
Time frame: At 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, 180, 195, 210 min
Food Intake
Assessed by ad'libitum meal after the 210 min GLP-1 infusion / glucose clamp.
Time frame: at 210 min
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