Pharmacokinetics of Vancomycin for Inhalation in Cystic Fibrosis

Inclusion Criteria: * Male or female ≥ 18 years of age. * Confirmed diagnosis of CF based on the following criteria: * positive sweat chloride \> 60 mEq/liter (by pilocarpine iontophoresis) and/or * a genotype with two identifiable mutations consistent with CF or abnormal NPD, and * one or more clinical features consistent with the CF phenotype. * Chronic sputum producer able to spontaneously produce sputum * FEV1 \> 40% of predicted normal for age, gender, and height * Previous use of any inhaled antibiotics within the last year * Ability to provide written informed consent * Ability to adhere to the protocol Exclusion Criteria: * Use of inhaled or intravenous vancomycin within two weeks of the study visit * Known history of intolerance to inhaled vancomycin or inhaled albuterol. * Known history of hypersensitivity to vancomycin or other glycopeptide antibiotics * History of sputum culture with Burkholderia cepacia complex in the last two years. * Pregnancy * Woman who are lactating and not willing to stop nursing on the day of the study visit and the subsequent 48 hours. * Current use of oral corticosteroids in doses exceeding the equivalent of 10mg of prednisone a day or 20mg of prednisone every other day. * Patients not willing to hold other inhaled antibiotics (for example TOBI, Cayston, or Colistin) for at least 2 days prior to the study visit. * Patients not willing to hold loop diuretics (i.e. furosemide, torsemide, ethacrynic acid) on the morning of the study visit. * History of ABPA or reactive airways disease that has required treatment within the last year. * Creatinine greater than 2.0 mg/dL within the last year. * Oxygen saturation ≤ 92% on room air. * History of patient reported hearing loss * Any serious or active medical or psychiatric illness, which in the opinion of the investigator, would interfere with patient treatment, assessment, or adherence to the protocol. * History of or listed for solid organ or hematological transplantation