Preeclampsia: A Marker for Future Cardiovascular Risk in Women

Inclusion Criteria: 1. Healthy female premenopausal volunteers, with regular menstrual cycles (at least 8 cycles per year) 2. Age 21 to 50 years 3. Systolic blood pressure \<140 and \>90 mmHg and diastolic blood pressure \<90 and \>60 mmHg at the screening visit 4. Body mass index \< 35 kg/m2 5. History of normotensive pregnancy or history of preeclamptic or hypertensive pregnancy within the last 10 years 6. No clinically significant abnormalities on screening tests (complete blood count, serum electrolytes, liver enzymes, thyroid stimulating hormone, urinalysis, and electrocardiogram) Exclusion Criteria: 1. Current pregnancy 2. Lactation 3. Elevated blood pressure (systolic blood pressure \> 140 mmHg or diastolic blood pressure \> 90 mmHg) 4. Current diagnosis of hypertension 5. Current diagnosis of diabetes mellitus 6. Personal history of coronary disease, stroke and kidney disease 7. Use of prescription medications (with the exception of stable thyroid hormone replacement dose) within 2 weeks of study 8. Use of oral contraceptives or other hormone therapy within 3 months of study; 9. Renal impairment (estimated GFR\<60) 10. Active liver disease (AST, ALT, alkaline phosphatase \> 1.5 times normal); 11. Current smoking, defined as smoking within the 6 months before the screening visit 12. Current or past recreational drug use