The primary objective of this study is to detect differences in anti-S. aureus antibody assays (IgM, IgA, IgG and its subclasses) between (1) a group of patients with bacteremia due to a chronic wound (cases) and (2) a group of patients with localized chronic wound infections (control).
Secondary objectives include: * To propose a diagnostic marker of bacteraemia based on the detection of anti-S. aureus by xMAP technology * To evaluate the kinetics of the humoral immune response to S. aureus in patients with bacteraemia * To evaluate the virulence of S. aureus isolated from chronic, infected wounds * To enrich the Nîmes University Hospital biobank * To evaluate the prevalence of different antibodies within each group
Study Type
OBSERVATIONAL
Enrollment
69
Antibody assays for S. aureus at Day 0, Day 2, Day 4, Day 7, Day 30 and Month 6.
Hemogramme + CRP on Days 0, 2, 4 and 7
CHU de Nîmes - Hôpital Universitaire Carémeau
Nîmes, Gard, France
CHU de Nîmes - Hôpital Universitaire de Réadaptation du Grau du Roi
Le Grau-du-Roi, France
CHU de Montpellier - Hôpital Saint-Eloi
Montpellier, France
CHU de Nantes - Hôtel-Dieu
Nantes, France
Humoral immune response
Humoral immune response via production of anti-staphylococci antibodies in patients with bacteraemia versus those with localized wound infections. 22 antibodies will be tested(quantitative determination by xMAP technology).
Time frame: 6 months
Antibody production kinetics
Kinetics of the production of the 22 antibodies against S. aureus in the 2 groups. The temporal evolution of antibody titres determined via xMAP technology will be followed throughout the study.
Time frame: 6 months
Presence / absence of clonal complexes of the S. aureus strains
determined by microarrays
Time frame: 6 months
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CHU de Nice - Hôpital Pasteur
Nice, France
CHU de Nice - Hôpitaux L'Archet 1 et 2
Nice, France