The Effect of Protein and Calcium on Weight Change and Blood Lipid Profile

Inclusion Criteria: * Both men and women can be included * All ethnic groups can be included * Age: 18≤age≤60 * BMI: 28≤BMI≤40 Exclusion Criteria: * smoking * use of cholesterol-lowering medications or other medications considered to be of importance for participation in the trial * use of special diets (eg. Atkins, vegetarian) within 2 months before the start of the project. * elite athletes or if subjecct is planning to become elite athlete (e.g. Planning major changes in physical activity during the experiment). * blood donation within the last 3 months before the commencement of the trial * weight change\> 3 kg within 2 months before the start of the project * sagital height of 32 cm * pregnant or nursing women or women planning to become pregnant within the next 12 months. * surgically treated obesity * participation in other clinical trials within the last 3 months * if consuming medicine daily, consumption of prescription medicine needs to have been stable through at least the last 3 months and is expected to remain so throughout the whole study. However, a subject cannot be included if the treatment includes medicine that is a systemic treatment that is considered to be of importance for participation in the project. * alcohol or drug use (based on clinical judgment) * subjects who are unable to give an informed consent. * chronic systemic infectious or inflammatory disorders * chronic endocrine disorders * inadequate nutrient uptake, or chronic gastrointestinal or liver diseases (apart from irritable bowel disorder) * cardiovascular disease, recognized heart failure or brain disease * cancer within the past 10 years * subject who is judged unable to participate in an LCD (low calorie diet) in an 8 week period * known allergy to para-aminobenzoic acid (PABA) * subjects who are in a general physical and/or mental condition,where the researcher assesses that the subject does not conform with the general aim of the study. * subjects with a hemoglobin value below 7 mol / L. Those with a hemoglobin value below 8 mol / L (measured at screening) cannot volunteer for the meal test during hood measurements, but may be included in the main study. When starting on a weight maintenance period: • persons during weight loss period, loss of \<8% of their initial body weight