The purpose of the present study is to conduct a thorough and relevant physiology study of carriers and non-carriers of the gene variant X in order to determine the effect of the genetic variant on various metabolic parameters.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
40
FPIR: intravenous infusion of 20 % glucose 0.3 mg/kg in 2 minutes followed by blood sampling at times 0, 2, 4, 6, 8, and 10. Duration 10 minutes. HEC: intravenous infusion of actrapid 1mU/kg/minute, simultaneous infusion of 20 % glucose at variable rate to reach plasma blood glucose level of 5 mmol/L. Duration 120 minutes Glucose tracer: bolus of 3H3glucose (12µCi) followed by infusion of 3H3glucose (0,12 µCi/min). Duration 120 minutes. Palmitate tracer:\[9,10-3H\]-palmitate 0,3 µCi/min. Duration 60 minutes.
Medicinsk forskningslaboratorium, Aarhus Universitet
Aarhus, Denmark
insulin secretion
Estimation of first phase insulin secretion
Time frame: 10 minutes
Insulin resistance
Estimation of insulin resistance using hyperinsulinemic euglycemic clamp and glucose and lipid tracers
Time frame: 240 minutes
body composition
Evaluation of body composition using Dxa scan and MRI scan
Time frame: 60 minutes
atherosclerosis
Evaluation of presence and severity of atherosclerosis using ultrasound scan of the common carotide artery
Time frame: 30 minutes
biochemical blood profiling
Various tests run on blood samples
Time frame: at baseline
Insulin resistance
Biopsi from muscle and adipose tissue performed at baseline and during clamp study.
Time frame: 60 minutes
Blood pressure
Measurement of blood pressure every 20. minutes/24 hours
Time frame: 24 hours
Indirect calorimetry
Estimation of resting energy expenditure and respiratory quotient
Time frame: 60 minutes
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