In this study, the investigators are interested in assessing the effects of the isocaloric increase of eating frequency on appetite and metabolism. How the consumption of an isocaloric breakfast in four intakes vs. one can modify satiety and appetite control in lean and obese subjects through : * the physiological consequences : difference in postprandial kinetics of glucose, non esterified fatty acid, triglyceride, the secretion of satiety gut hormone (insulin, ghrelin, leptin and cholescystokinine (CCK), peptide YY (PYY), glucagon-like peptide-1 (GLP-1), nutrients oxidative fate and plasmatic oxidative stress (Malondialdehyde (MDA), glutathion, lipid hydroperoxides) * eating behavior during an ad libitum buffet test meal
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
37
The subjects receive the same breakfast with two sequences : * breakfast in one intake at 8:00 * breakfast in four isocaloric intakes (8:00, 9:00, 10:00, 11:00) The sequence made twice : one for metabolic study and one for the study of eating behavior The lunch during metabolic study is a standard meal The lunch during behavioural study is an ad libitum buffet test meal. The administration order is determined by randomized allocation. The wash-out period is one to three weeks. There are not diet or exercise interventions.
Centre Hospitalier Lyon Sud - Centre de Recherche en Nutrition Humaine
Pierre-Bénite, France
Ghrelin plasmatic concentration
Time frame: 240 minutes after breakfast beginning (just before the lunch)
Satiety through food intakes (quantitative) at the ad libitum buffet test meal
Time frame: Since the beginning of the buffet test meal (240 minutes after breakfast beginning) to the end of the meal (at the latest 270 minutes after breakfast beginning).
Satiety through visual analogue scale
Time frame: 240 minutes after breakfast beginning (just before the lunch)
Plasma metabolite concentrations (glycaemia, non esterified fatty acid,
Time frame: kinetics during 430 minutes
Endocrine concentrations (insulin, ghrelin, GLP-1, PYY, leptin, C-peptide)
Time frame: kinetics during 430 minutes
Plasma markers of oxidative stress (MDA, gluthation, lipid hydroxide)
Time frame: kinetics during 430 minutes
Lipid oxidation
Time frame: kinetics during 430 minutes
Eating behaviour at the buffet test meal by video recording (food choice, nutritional composition of the meal , kinetics of nutrient intake, meal structure, lunch duration)
Time frame: during the buffet test meal (max : 30 minutes)
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