Acute Effects of Wine Consumption on Healthy Volunteers

Harokopio University10 enrolled

Overview

The purpose of this study is to investigate whether red and white wine consumption has acute effects on postprandial biochemical markers related to platelet aggregation, inflammation and oxidative stress compared to water or 12.5% ethanol aqueous solution consumption.

The last few years, epidemiologic studies indicate that regular moderate consumption of alcohol is associated with lower risk of coronary heart disease and heart attack, as well as with lower mortality. More specific, a J or U-shaped association between alcohol consumption and the incidence of coronary heart disease have been suggested, which means that there was lower disease risk in moderate alcohol consumers than in abstainers or heavy drinkers. The scientific interest was focused on wine after the term "French paradox" was introduced, in order to describe the epidemiological observation that the French suffer a relatively low incidence of coronary heart disease, despite having a diet relatively rich in saturated fats. The paradox was attributed to the moderate consumption of red wine by French. Even though many clinical studies have occurred since then, only few of them report the postprandial effect of wine, mainly focusing on the study of oxidative stress markers and endothelium dysfunction. Also, a limited number of publications refer to the postprandial wine effect upon platelet aggregation, which is an indicative marker for inflammation / thrombosis and atherosclerosis. The limited clinical evidence prompted us to investigate the postprandial effect of wine consumption upon platelet aggregation, inflammation and oxidation markers, by undertaking a clinical study of crossover design. The subjects randomly consumed 4ml of drink \[Robola or Cabernet Sauvignon or 12.5% ethanol or water\]/kg of individual, parallel with a standardized meal, which consisted of 30.8% carbohydrates, 12.0% proteins and 53.1% fat. The meal total energy was 787.2 kcal.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

PREVENTION

Masking

SINGLE

Enrollment

Conditions

Healthy, Postprandial

Interventions

Robola, Cabernet Sauvignon winesOTHER

4 treatments on separate days: the subjects randomly consumed 4ml of drink \[white wine or red wine or 12.5% ethanol or water\]/kg of individual, parallel with a standardized meal.

Eligibility

Sex: MALEMin age: 26 YearsMax age: 39 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * healthy * non-obese Exclusion Criteria: * smokers * those who reported slimming or any other dietary regime * abstainers from alcohol consumption * heavy drinkers * athletes * subjects who were on medication, such as aspirin, that may have an impact on platelet aggregation or surgical events that may have affected the study outcomes * participants with a known diagnosis of either hypertension or diabetes * subjects on medication

Locations (1)

Department of Nutrition-Dietetics, Harokopio University

Athens, Greece

Outcomes

Primary Outcomes

platelet aggregation

In each time point platelet rich plasma (PRP) was isolated from the blood of volunteers and platelet aggregation upon Platelet activating factor (PAF) was measured in CHRONO-LOG aggregometer.

Time frame: baseline

platelet aggregation

In each time point platelet rich plasma (PRP) was isolated from the blood of volunteers and platelet aggregation upon Platelet activating factor (PAF) was measured in CHRONO-LOG aggregometer.

Time frame: 30 min after standardized meal plus tested drink consumption.

platelet aggregation

In each time point platelet rich plasma (PRP) was isolated from the blood of volunteers and platelet aggregation upon Platelet activating factor (PAF) was measured in CHRONO-LOG aggregometer.

Time frame: 90 min after standardized meal plus tested drink consumption.

platelet aggregation

In each time point platelet rich plasma (PRP) was isolated from the blood of volunteers and platelet aggregation upon Platelet activating factor (PAF) was measured in CHRONO-LOG aggregometer.

Time frame: 150 min after standardized meal plus tested drink consumption

platelet aggregation

In each time point platelet rich plasma (PRP) was isolated from the blood of volunteers and platelet aggregation upon Platelet activating factor (PAF) was measured in CHRONO-LOG aggregometer.

Time frame: 210 min after standardized meal plus tested drink consumption.

platelet aggregation

In each time point platelet rich plasma (PRP) was isolated from the blood of volunteers and platelet aggregation upon Platelet activating factor (PAF) was measured in CHRONO-LOG aggregometer.

Time frame: 300 min after standardized meal plus tested drink consumption.

Data from ClinicalTrials.gov

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markers of inflammation

Time frame: baseline

markers of inflammation

Time frame: 0 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 30 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 60 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 90 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 120 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 150 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 180 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 210 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 240 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 300 min after standardized meal plus tested drink consumption

markers of inflammation

Time frame: 360 min after standardized meal plus tested drink consumption

markers of oxidative stress