Comparison of Three Plasmodium Falciparum Isolates in a Controlled Human Malaria Infection

Inclusion Criteria: * 18-35 year-old healthy volunteers (males and females) * General good health based on history,clinical examination and basic haematology and biochemistry results * Negative pregnancy test in females * Use of adequate contraception for females * All volunteers must sign the informed consent form following proper understanding of the design and procedures of the study * Volunteer agrees to inform his/her general practitioner and agrees to sign a request for medical information concerning possible contra-indications for participation in the study * Willingness to undergo a Plasmodium falciparum sporozoite challenge * Agreement to stay in a hotel room close to the trial center during a part of the study (day 5 post-infection until three days after initiation of treatment) * Reachable by mobile phone during the whole study period * Available to attend all study visits * Agreement to refrain from blood donation to (Sanquin) blood bank or for other purposes, during the course of the study and for a minimum of three years thereafter * Willingness to undergo an HIV, HBV and HCV test * Negative urine toxicology screening test at screening visit and on the day before challenge * Willingness to take a curative regimen of Malarone® Exclusion Criteria: * History of malaria * Plans to travel to endemic malaria areas during the study period * Previous participation in any malaria vaccine study and/or positive serology for P. falciparum * Symptoms, physical signs and laboratory values suggestive of systemic disorders, including but not limited to renal, hepatic, cardiovascular, pulmonary, skin, immunodeficiency, psychiatric and other conditions, which could interfere with the interpretation of the study results or compromise the health of the volunteer during infection * History of diabetes mellitus or cancer (except basal cell carcinoma of the skin) * Clinically significant ECG abnormalities at screening, or history of arrhythmia's or prolonged QT-interval * Positive family history of cardiac disease in 1st or 2nd degree relatives \<50 years old * An estimated ten year risk of fatal cardiovascular disease of ≥5%, as estimated by the Systematic Coronary Risk Evaluation (SCORE) system * Body Mass Index (BMI) below 18 or above 30kg/m2 * Any clinically significant deviation from the normal range in biochemistry or haematology blood tests or in urine analysis * Positive HIV, HBV or HCV tests * Participation in any other clinical study during or within 30 days prior to the onset of the trial * Pregnant or lactating women * Volunteers unable to give written informed consent * Volunteers unable to be closely followed for social, geographic or psychological reasons * Previous history of drug or alcohol abuse interfering with normal social function during a period of one year prior to enrolment in the study * A history of psychiatric disease or convulsions * Known hypersensitivity to anti-malarial drugs * History of severe reactions or allergy to mosquito bites * The use of chronic immunosuppressive drugs, antibiotics, or other immune modifying drugs within three months before study onset (inhaled and topical corticosteroids are allowed) or during the study period * Contra-indications for Malarone® use including treatment taken by the volunteers that interfere with Malarone® * Any confirmed or suspected immunosuppressive or immunodeficient condition, including asplenia * Co-workers of the department of Medical Microbiology of the UMC St Radboud or Havenziekenhuis, Rotterdam * A history of sickle cell, thalassaemia trait and G6PD deficiency