The Effect of Calorie Deprivation on Adipose Tissue Distribution

Inclusion Criteria: * Men and women, ages 21-45 years * 101-130% of ideal body weight as defined by the 1983 Metropolitan Life Insurance Height and Weight Tables * Normal thyroid function * Regular menses (women) * Normal liver transaminases Exclusion Criteria: * Any disease known to affect bone metabolism, including untreated thyroid dysfunction, Cushing's syndrome, or renal failure * Any medication known to affect bone metabolism -- including systemic steroids or immunosuppressants -- within three months of the study, excluding estrogen and progestins. Patients receiving depot medroxyprogesterone (Depo-Provera) will be excluded from participating for six months after their last injections. Bisphosphonates must have been discontinued for at least one year before participation * Serum potassium \<3.0 meq/L * Pregnant and/or breastfeeding (women) * Diabetes mellitus * Active substance abuse, including alcohol * Contraindications to MRI: cardiac pacemaker, metal implants, claustrophobia