The overall purpose of this study is to enhance identification and isolation of ADSCs and adipocytes, to find the optimum culturing conditions to increase growth and volume of grafts, and to study the effects of cryopreservation on ADSCs and adipocytes. Fat injections are used in aesthetic surgery but tend to disassemble over time. If the investigators can enhance growth and volume of fat in culture, the investigators could use fat grafts in reconstruction of cancer and traumatic injury defects with the patients own cultured fat cells and grafts.
Study Type
OBSERVATIONAL
Enrollment
240
University of Nevada School of Medicine Patient Care Center
Las Vegas, Nevada, United States
RECRUITINGPercent of Viable ADSCs
Percent of viable ADSCs will be measured on fresh samples and samples refrigerated for different times.
Time frame: Day of Surgery
Percent of Apoptotic ADSCs
Percent of apoptotic ADSCs will be measured on fresh samples and on refrigerated samples at 1, 2, 3, and 4 days refrigerated storage.
Time frame: Day of Surgery
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