Inadequate suppression of intramuscular and adipose tissue lipolysis, and consequent excessive delivery of fatty acids to ectopic tissues (e.g. muscle, pancreas and liver) could play an important role in the development and exacerbating of insulin resistance. Therefore, the investigators propose to study the regulation of adipose tissue and skeletal muscle lipolysis, as well as further characterize the intracellular lipolytic pathways within these tissues, in obese normoglycaemic versus long-term diagnosed type 2 diabetic subjects.
Study Type
OBSERVATIONAL
Enrollment
20
Department of Human Biology, Maastricht University Medical Centre
Maastricht, Netherlands
Change from baseline in insulin sensitivity of adipose tissue and skeletal muscle lipolysis
Changes from baseline in glycerol (lactate, pyruvate and glucose) in tissue microdialysate during a 3 step euglycemic hyperinsulinemic clamp (8, 20 and 40mU of insulin), every step for 2h
Time frame: 8, 20 and 40 mU insulin (2 hours)
Baseline adipose tissue and skeletal muscle gene expression
gene expression of genes related to lipid, glucose metabolism, insulin signalling will be measured in baseline adipose tissue and skeletal muscle biopsies
Time frame: baseline
Baseline skeletal muscle lipid accumulation
Baseline skeletal muscle lipid accumulation is measured (TAG, DAG, Phospholipid and fatty acids)
Time frame: Baseline
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