It has been evidenced that chocolate and cocoa consumption increase vasodilation and reduce blood pressure. However, the mechanisms implicated in these effects have not been elucidated yet. The purpose of this study is to evaluate changes in gene expression induced by the administration of a polyphenol-rich cocoa extract in peripheral blood mononuclear cells (PBMC) in humans.
This is a prospective, randomized, controlled, double-blind and cross-over study. The intervention will consist in the administration of 250g of chocolate cream with or without the extract after 12 hours fasting. Blood samples will be taken at point 0, 2, 3 and 6 hours after the administration. At each time point, blood pressure and endothelial function (with a non-invasive method) will be measured.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
25
250 g of chocolate cream supplemented with 2.3 g of polyphenol-rich cocoa extract (containing 1050 mg of total polyphenols)
Technological Center of Nutrition and Health (CTNS)
Reus, Tarragona, Spain
Gene expression changes in PBMCs (using a microarray)
Time frame: 0-6 hours
Blood pressure
Time frame: 0-2-3-6 hours
Endothelial function (assessed by ischemic reactive hyperemia)
Time frame: 0-2-3-6 hours
Plasma levels of procyanidins metabolites
Time frame: 0-6 hours
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.