Electroporation will increase the efficiency of DNA priming in terms of immune responses and will lead to a dose sparing DNA vaccine regimen. Furthermore increased DNA vaccine concentration will reduce the number of shots necessary to deliver the full dose and induce comparable immune responses as with lower DNA vaccine concentrations.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Masking
QUADRUPLE
Enrollment
198
Instituto Nacional de Saude
Maputo, Mozambique
Muhimbili University of Health and Allied Sciences
Dar es Salaam, Tanzania
Mbeya Medical Research Programme
Mbeya, Tanzania
The presence of an interferon gamma ELISpot responses to a pool of HIV peptides encoded by the vaccine to which there was no response at baseline
Time frame: 2 weeks after the last vaccination
Grade 3 or above local and systemic solicited adverse events
Time frame: Within 2 weeks post each immunization up to week 64 from enrollment
The presence of CD4+ and CD8+ T-cell cytokine responses to pools of HIV peptides assessed by Intracellular cytokine staining
Time frame: 2 weeks post last vaccination
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