fMRI Evaluation of Pain Central Sensitization Phenomena in Subjects With Knee Osteoarthritis

Inclusion Criteria: * Patients: 1. A diagnosis of knee OA and suitable for the study as determined by a responsible physician, based on a medical evaluation including medical history, physical examination, laboratory tests and cardiac monitoring. 2. Have a radiological and clinical diagnosis of knee OA based upon American College of Rheumatology (ACR) criteria (1986) affecting at least one knee of a minimum of 3 months in symptom duration prior to screening. 3. Subject is either male or female and at least 45 years of age. 4. A minimum of 4 out of 10 on the numerical rating scale (Brief Pain Inventory- BPI item 5) at screening and/ or a requirement for the use of an analgesic for the pain in the OA knee. In addition, baseline pain must be stable for at least 72 hours prior to fMRI assessment or fMRI assessment may be delayed. 5. A maximum of 8 out of 10 on the NRS at screening. Enrolment may be delayed if, in the opinion of the investigator, the severity of pain represents an isolated incident. 6. A female subject is eligible to participate if she is of non-childbearing potential. 7. Body weight \<120kg. 8. Capable of giving written informed consent, which includes compliance with the requirements and restrictions listed in the consent form. 9. Subject demonstrates an understanding of the study and a willingness to participate as evidenced by voluntary written informed consent. * Controls: 1. Subject is either male or female and at least 45 years of age. 2. Absence of clinically relevant neurologic and psychiatric disease and history of head trauma with loss of consciousness. 3. Absence of non-compensated medical diseases and pain disorders that may relevantly affect brain function and pain perception. 4. No clinical evidence of knee OA. 5. A female subject is eligible to participate if she is of non-childbearing potential. 6. Body weight \<120kg. 7. Capable of giving written informed consent, which includes compliance with the requirements and restrictions listed in the consent form. 8. Subject demonstrates an understanding of the study and a willingness to participate as evidenced by voluntary written informed consent. Exclusion Criteria: 1. \- Subjects will not be permitted to heavily exercise for 12 hours prior to fMRI assessment 2. \- Subjects will be required to refrain from the following treatments: I.- Analgesic and anti-inflammatory drugs for 3 days prior to fMRI assessment except for rescue medication. Rescue medication will be based on paracetamol (dosed 1g/8h), which in any case must be withdrawn for a minimum of 24 h prior to fMRI assessment or the fMRI assessment may be delayed. II.- Steroid injections into the joint or muscle for three months prior to fMRI assessment III.- Hyaluronan injections into index knee within the previous 6 months prior fMRI assessment IV.- Non-pharmacological treatments, such as heat wraps or massage for 24 hours prior to fMRI assessment. V.- Antidepressants not allowed: MAOIs, Tricyclic antidepressants, mixed 5HT \& NE reuptake inhibitors (eg. Duloxetine). SISR are not allowed as a recent prescription, moreover they are allowed in case of chronic use (more than 6 weeks). If those drugs are used for less than 6 weeks, patients could participate in the study after a washout period. VI.- Anticonvulsant indicated as a pain killer are not allowed, but patients could participate in the study after a washout period. VII.- Patients can take low doses of hypnotic drugs (eg. Lorazepam 1mg/OD or Diazepam 5mg/OD) if they are used as a chronic prescription (more than 6 weeks). If they are used for less than 6 weeks, patients could participate in the study after a washout period. Subjects will be allowed to take their chronic (non-analgesic and non-anti-inflammatory) medications if the treatments have been used continuously for at least six weeks prior to assessment.