Postprandial IHL and IMCL Measured With Proton Magnetic Resonance Spectroscopy

Maastricht University Medical Center9 enrolled

Overview

The major research objective is to assess the potential of Proton Magnetic Resonance Spectroscopy to follow Intrahepatic lipid and Intramyocellular lipid dynamics after a single meal. Furthermore we aim to investigate whether the addition of protein to a high fat breakfast results in a reduced postprandial accumulation of lipids when compared to a high fat breakfast alone.

Fat accumulation in non-adipose tissue, such as liver and muscle, is associated with reduced insulin sensitivity and cardiovascular disease. Little is known about the dietary determinants and the time course of this ectopic fat accumulation. Therefore the general aim of this project is to develop and validate methodology to follow postprandial hepatic and muscular lipid retention. Proton Magnetic Resonance Spectroscopy (1H-MRS) is a well-established tool for noninvasive quantification of intrahepatic lipids (IHL) and intramyocellular lipids (IMCL). Previous studies have suggested a relatively rapid regulation of IHL and IMCL levels, indicated by changes in these total lipid pools after high fat diets of 3 or 4 days. Although IHL and IMCL levels seem to change rapidly, data on IHL and IMCL levels after a single meal are lacking.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Postprandial IHL and IMCL Dynamics

Interventions

High fat breakfastDIETARY_SUPPLEMENT

The HF breakfast will consist of sausage rolls ("saucijzenbroodjes"), available at the local Albert Heijn (Albert Heijn B.V., Zaandam, The Netherlands).

HF breakfast with proteinDIETARY_SUPPLEMENT

For the HFP breakfast, the subjects will consume 'Protifar' (Nutricia, Cuijk, The Netherlands) in addition to the sausage rolls.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Age 18-40 years * Healthy * Stable dietary habits * No use of medication * Lean subjects: BMI 18-25 kg/m2 * Obese subjects: BMI 30-35 kg/m2 Exclusion Criteria: * Any medical condition requiring treatment and/or medication use * Alcohol consumption of more than 20 g per day (± 2 units) * Smoking * Unstable body weight (weight gain or loss \> 3 kg in the past three months) * Participation in another biomedical study within 1 month prior to the screening visit * Contraindications for MRI scan: * Central nervous system aneurysm clips * Implanted neural stimulator * Implanted cardiac pacemaker of defibrillator * Cochlear implant * Iron- containing corpora aliena in the eye or brain * Hearing aids and artificial (heart) valves which is contraindicated for MRS * Claustrophobia

Locations (1)

Maastricht University Medical Center +

Maastricht, Netherlands

Outcomes

Primary Outcomes

Difference between pre- and postprandial IHL and IMCL

The primary research objective is to assess the potential of 1H-MRS to follow IHL and IMCL dynamics after a single meal in lean and obese subjects.

Time frame: 3 and 5 hours after high fat breakfast

Secondary Outcomes

Difference between postprandial IHL and IMCL in high fat and high fat with extra protein breakfast

We aim to investigate whether the addition of protein to a high fat breakfast (HFP) results in a reduced postprandial accumulation of lipids when compared to a high fat breakfast (HF) alone.

Time frame: 3 and 5 hours after high fat breakfast

Data from ClinicalTrials.gov

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