A Molecule Basic Study of Early Warning for New Pathogenic Risk of Ankylosing Spondylitis

Inclusion Criteria: 1. Aged from 16-65 years, sign the Informed Consent 2. Fulfill 1984 modified NewYork classification criteria for AS 3. Have an active refractory disease defined by a score ≥40 on the Bath AS Disease Activity Index (BASDAI) (0-100) despite optimal non-steroidal anti-inflammatory drug (NSAID) treatment. 4. Commitment to contraceptive for woman Exclusion Criteria: 1. Completely stiff spine 2. Received spinal or joint surgery within 2 months 3. Received anti-TNF therapy within 3 months 4. History of the listed diseases: heart failure, Multiple sclerosis, severe chronic obstructive pulmonary disease, frequent infections, lymphoma or other cancers, tuberculosis 5. Female of pregnancy or breast feeding 6. Hb≤ 9g/dl for male or Hb ≤ 8.5 g/dl for male, ALT/AST≥2folds of upper level normal range, Creatine≥120mol/L(≤1.4mg/dl)