Evaluating Modes of Influenza Transmission

Inclusion Criteria: * Body Weight: A total body weight ≥50 kg and a Body mass index (BMI) \>18 (if BMI is \>32, a body fat percentage within WHO and NIH range for gender and age. BMI \[kg/m2\] = Body weight \[kg\] ÷ Height2 \[m2\] * Contraception: Nonsterilised males must agree to refrain from fathering a child from the point of entering quarantine until the Day 28 follow up visit. Use of one effective form of contraception is acceptable. Sexually active females of childbearing potential must agree to use 2 effective methods of avoiding pregnancy that are deemed to be effective from the point of entry into the quarantine unit until the Day 28 follow up visit. Acceptable forms of effective contraception include: 1. Established use of oral, injected or implanted hormonal methods of contraception. 2. Placement of an intrauterine device (IUD) or intrauterine system (IUS). 3. Barrier methods of contraception: Condom or Occlusive cap (diaphragm or cervical/vault caps) with spermicidal foam/gel/film/cream/suppository. 4. Male sterilisation (with the appropriate postvasectomy documentation of the absence of sperm in the ejaculate). \[For female subjects on the study, the vasectomised male partner should be the sole partner for that subject\]. 5. True abstinence: When this is in line with the preferred and usual lifestyle of the subject. \[Periodic abstinence (e.g., calendar, ovulation, symptothermal, postovulation methods) and withdrawal are not acceptable methods of contraception\]. * Informed Consent: An informed consent document signed and dated by the subject and investigator * Serosuitability: Serosuitable for challenge virus Exclusion Criteria: * Smoking: Significant history of any tobacco use at any time (≥ total 10 pack year history, eg. one pack a day for 10 yrs) * Pregnancy/Lactation: Subjects who are pregnant or nursing, or who have a positive pregnancy test at any point in study * Previous Medical History: Presence of significant acute or chronic, uncontrolled medical illness, that in the view of Investigator(s)is associated with increased risk of complications of respiratory viral illness * Pulmonary Function: Abnormal pulmonary function in the opinion of the investigator evidenced by clinically significant abnormalities in spirometry * Immune: History or evidence of autoimmune disease or known impaired immune responsiveness (of any cause) * Asthma: History of asthma, COPD, pulmonary hypertension, reactive airway disease, or any chronic lung condition of any aetiology.History of childhood asthma until and including the age of 12 is acceptable. * HIV \& Hepatitis: Positive HIV, hepatitis B (HBV), or hepatitis C (HCV) antibody screen. * Anatomic abnormalites of nasopharynx:Significant abnormality altering anatomy of nose or nasopharynx * Epistaxis: Clinically significant history of epistaxis * Nasal Surgery: Any nasal or sinus surgery within 6 months of inoculation * Fainting: Recent (within the last 3 years of the screening visit) and/or recurrent history of clinically significant autonomic dysfunction (e.g. recurrent episodes of fainting, palpitations, etc) * Lab Test/ECG: Laboratory test or ECG which is abnormal and deemed by investigator(s) to be clinically significant. * Drugs of abuse etc: Confirmed Positive test for class A drugs or alcohol that cannot be satisfactorily explained * Venous Access: Venous access deemed inadequate for phlebotomy (and IV infusion) demands of study * Hayfever: Subjects symptomatic with hayfever on admission or prior to inoculation. * Allergies: Any known allergies to excipients in challenge virus inoculum * Healthcare workers: Health care workers (including doctors, nurses, medical students and allied healthcare professionals) anticipated to have patient contact within two weeks of viral challenge. Healthcare workers should not work with patients until 14 days after challenge or until symptoms are fully resolved (whichever is longer). In particular, health care workers who work in units housing, elderly, disabled or severely immunocompromised patients (e.g. bone marrow transplant units) will be excluded. * Household members: Presence of household member or close contact (for an additional 2 weeks after discharge from the isolation facility) who is: 1. less than 3 years of age 2. any person with any known immunodeficiency 3. any person receiving immunosuppressant medications 4. any person undergoing or soon to undergo cancer chemotherapy within 28 days of viral inoculation 5. any person with diagnosed emphysema or chronic obstructive pulmonary disease (COPD), is elderly residing in a nursing home, or with severe lung disease or medical condition that may include but not exclusive to conditions listed (detailed in protocol); or 6. any person who has received a transplant (bone marrow or solid organ) * Travel: Intending to travel within next 3 months (to countries for which travel vaccinations are recommended). * Employers: Those employed or immediate relatives of those employed at RVL or staff/ students working directly for any unit in which CI works. * Blood loss/receipt: Receipt of blood or blood products, or loss (including blood donations) of 450 mL or more of blood, during the 3 months prior to inoculations. * Use of nasal congestion products - acute/chronic: Acute use i.e. within 7 days prior to viral challenge of any medication or other product (prescription or OTC), for symptoms of hayfever, rhinitis, nasal congestion or respiratory tract infection. * Other IMP or virus challenges: Receipt of any investigational drug within 3 months prior inoculation. Receipt of more than 4 investigational drugs within the previous 12 months. Prior participation in a clinical trial with same strain of respiratory virus. Participation in other respiratory virus challenge within 1 year prior to challenge * Chemotherapy: Receipt of systemic glucocorticoids, antiviral drugs, and immunoglobulin's (Igs) or other cytotoxic or immunosuppressive drug within 6 months prior to dosing. Receipt of any systemic chemotherapy agent at any time. * Current or recent respiratory infection: Presence of significant respiratory symptoms on day of challenge or between admission for challenge and challenge with/exposure to virus. History suggestive of respiratory infection within 14 days prior to admission for challenge exposure. * General screening: Any other finding in medical interview, physical exam, or screening investigations that, in the opinion of the investigator, GP or sponsor, deem subject unsuitable for the study.