Incretin-based therapies have shown significant effects beyond those on glucose metabolism. We aim in the present study to evaluate the effects of liraglutide on several cardio-metabolic risk markers.
In details, we will evaluate the effects of liraglutide on carotid-intima media thickness, oxidative stress, ghrelin, heat shock proteins and lipoproteins.
Study Type
OBSERVATIONAL
Enrollment
300
University Hospital of Palermo
Palermo, Italy
carotid intima-media thickness
carotid intima-media thickness will be assessed by doppler ultrasonography
Time frame: every four months for a total period of 2 years
changes in oxidative stress markers
Serum LOOH levels were evaluated following the oxidation of Fe2+ to Fe3+ in the presence of xylenol orange at 560 nm. Levels of GSH were measured in serum using a spectrophotometric assay based on the reaction of thiol groups with 2,2-dithio-bis-nitrobenzoic acid, while the production of ROS was assessed using fluorescent probe dihydroethidium staining (Sigma).
Time frame: after 2 months of therapy
changes in plasma ghrelin concentrations and heat-shock proteins
plasma ghrelin concentrations and heat shock protein 60 were measured by ELISA
Time frame: after 2 months of therapy
changes in atherogenic lipoproteins
the full LDL subclass profile (from larger and less atherogenic LDL-1 and LDL-2, to smaller denser LDL-3 to LDL-7) was assessed by gel electrophoresis (Lipoprint, Quantimetrix, USA)
Time frame: after 2 and after 4 months of therapy
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