Biomarkers Before and After Nephrectomy of Locally Advanced or Metastatic Renal Cell Carcinoma Treated With Everolimus

Inclusion Criteria: * Advanced RCC or a resectable renal tumor and at least one measurable inoperable metastasis (at least 1 cm), in whom anti-angiogenic therapy is indicated * Patients without target lesions, with bone metastasis * Histologically confirmed clear cells RCC and possibility of adequate tumor sampling prior to treatment * No prior systemic treatment for RCC * Male or female, at least 18 years * PS ECOG 0-1 * Life expectancy at least 3 months * Adequate organ function with the following criteria: * Total serum bilirubin equal or less than 2 x ULN (Gilbert's disease exempted) * Serum transaminases and alkaline phosphatases equal or less than 2.5 x ULN, or in case of liver or bone metastasis equal or less than 5x ULN * Serum creatinine equal or less than 2 x ULN, creatinine clearance at least 50 ml/min * Absolute neutrophil count (ANC) at least 1500/mm3 * Platelets at least 100,000/mm3 * Hemoglobin at least 10.0 g/dL * INR equal or less than 1.7 or prothrombin time (PT) equal or less than 6 sec * Blood glucose less than 1.5x ULN * Fasting cholesterol equal or less than 5 mmol/L, triglycerides equal or less than 200 mg/dl, * Negative pregnancy test within 7 days prior to enrollment * Signed and dated IRB/ICE-approved informed consent form * Willingness and ability to comply with scheduled visits, treatment plans, laboratory tests and other study procedures. * Patient covered by the national health system Exclusion Criteria: * Previous nephrectomy * Histology: any histologic type different than ccRCC * Treatment in a clinical trial in the last 30 days * Previous treatment with everolimus or other mTOR-inhibitors and anti-angiogenic drugs * Any of the following within 12 months prior to treatment initiation: severe/unstable angina, myocardial infarction, coronary artery bypass graft, symptomatic congestive heart failure, ischemic or hemorrhagic stroke including transient ischemic attack, abnormal lung function. * Uncontrolled hypertension defined as systolic blood pressure \>150 mmHg or diastolic pressure \>90 mmHg, despite optimal medical treatment * Abnormal ECG (Clinically significant) * Treatment with vitamin K antagonists. Ongoing treatment with therapeutic doses of coumarin derivative anticoagulants (e.g. warfarin) or treatment within the 2 weeks before the first day of everolimus administration. Prophylaxis with low dose warfarin for deep vein thrombosis is permitted (up to 2 mg/day). Low molecular weight heparin is allowed. * Brain metastasis. (Brain scan or MRI is mandatory). Note: Previous treated brain metastasis (surgery ± radiotherapy, radiotherapy, radiosurgery or gammaknife) and satisfying the following three criteria are allowed: * Asymptomatic; * No evidence of any active brain metastasis 3 months prior inclusion; * No necessity of corticoid or antiepileptic treatment. * Pregnancy or breastfeeding. * Any second malignancy within the last 3 years with the exception of basal cell carcinoma, in situ cervical cancer and pT1/a bladder cancer with no evidence of recurrent disease for 12 months. * Clinically significant gastrointestinal abnormalities including but not limited to: * Malabsorption syndrome * Major resection of the stomach or small bowel that could affect the absorption of the study drug * Active peptic ulcer disease * Inflammatory bowel disease * Ulcerative colitis or other gastrointestinal conditions with increased risk of perforation * History of abdominal fistula, gastrointestinal perforation, or intra abdominal abscess within 28 days prior to beginning study treatment * Hepatitis B/C * Hypersensitivity to everolimus or any excipient of everolimus. * Any active (acute or chronic) or uncontrolled infection/disorders that impair the ability to evaluate the patient or for the patient to complete the study.