Effects of Free Fatty Acids and 3-hydroxybutyrate on Protein, Glucose, Lipid Metabolism and Intracellular Signals.

University of Aarhus10 enrolled

Overview

The investigators hypothesize that the investigators can detect protein-sparing effects of administration of the ketone 3-hydroxybutyrate and free fatty acids during simulation of an acute inflammatory disease. The investigators use the infusion of endotoxin, US standard reference E.coli in healthy subjects as a model for inflammation / infection and to evaluate the effect on protein metabolism using different tracers and the investigators can measure the various intracellular signaling pathways of selected muscle and adipose tissue.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Systemic Inflammatory Response Syndrome

Interventions

Eligibility

Sex: MALEMin age: 20 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * 20 \< body mass index \< 30 kg/m2. * written and orally informed consent before enrollment. Exclusion Criteria: * participation in other studies using radioactive tracers or other exposure to radiation (X-rays, scintigraphy etc). * allergy to soy products or eggs * diabetes, any type * epilepsy * ongoing infection * immune deficiency * cardiovascular disease * dysregulated hypertension * primary muscles disease, congenital or acquired

Outcomes

Primary Outcomes

Protein metabolism

Measurements of protein oxidation during intervention using radioactive tracer techniques.

Time frame: 6 hours intervention period

Secondary Outcomes

Activated intracellular signalling pathways

Measurements on biopsies from muscle and fatty tissue taken on day of study

Time frame: study day