The purpose of the study is to evaluate the bioavailability of acetate, propionate and butyrate in healthy humans using a stable isotope technology. In addition the level of acetate, propionate and butyrate production from inulin will be determined using the principle of isotope dilution.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
12
400 mg of sodium acetate 1-13C (2 colon delivery capsules with 200 mg)
340 mg of sodium propionate 1-13C (2 colon delivery capsules with 170 mg)
990 mg sodium butyrate 1-13C (2 colon delivery capsules with 495 mg)
KU Leuven/ UZ Leuven
Leuven, Vlaams-Brabant, Belgium
Concentrations of the short chain fatty acids (acetate, propionate and butyrate) in plasma and urine samples
Participants performed each 4 test days and they were followed each test day for 12 hours, with sample collections at regular time points. Blood samples were collected every hour during the first 4 hours and afterwards every 20 minutes during 8 hours. Urine samples were collected during 24h in different fractions: 0-4h, 4-8h, 8-12h, 12-24h.
Time frame: 12 hours each test day and 4 test days/volunteer
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15 g of inulin dissolved in 200 ml of water