Influence of Preconceptional Obesity on the Myometrial Cells Differentiation Toward a Contractyl Phenotype

Centre Hospitalier Universitaire Dijon38 enrolled

Overview

The purpose of this study is to improve the understanding of cellular mecanism involved in delivery disorders in obese women, first by characterizing the hyperplasia phenomenon of myometrail cells induced by inflammatory stimulation (Il-1B), then by studying the role of blood mononuclear cells, of leptine and of obese women sera to interfere with the transition of myometrial cells toward hyperplasia phenotype.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Pregnancy Complication

Interventions

Myometrium biopsy and blood samplesOTHER

The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean. The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.

Eligibility

Sex: FEMALEMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Informed consent signed * Patient affiliated with a social security scheme * In front of for obstetrical reasons to give birth by caesarean section (scheduled caesarean section) * Or a woman who is not obese in labour and needs to undergo a cesarean section * No obvious infectious context * Age \> 18 years old Exclusion Criteria: * Obvious inflammatory or autoimmune diseases * Suspected or confirmed infectious context, including virale infection VIH, VHC, VHB * Coagulation disorders

Locations (2)

Maternité du CHU de Dijon

Dijon, Bourgogne-Franche-Comté, France

Clinique Sainte Marthe

Dijon, France

Outcomes

Primary Outcomes

Measuring the expression level of markers (Gama actine, Desmine, Connexine-43, and Ocytocine receptor) by Westernblotting

Demonstrate in vitro the capacity of a Il-1B stimulus to induce the differentiation of the myometrial cells toward a hyperplasia phenotype, and that the answer depends on whether this is cells from normal weight women or obese women before conception.

Time frame: up to 2 months

Data from ClinicalTrials.gov

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