Training Study to Characterize Biomarkers to Flu Vaccines

Inclusion Criteria: 1. Healthy male or female subjects aged 18-45 years inclusive. Randomisation will ensure equal numbers of men and women. 2. The subject is, in the opinion of the investigator, healthy on the basis of medical history, vital signs, and the results of routine laboratory tests with no active disease process that could interfere with the study endpoints. 3. Body Mass Index ≥18.5 and \<29.5 4. The subject is able to read and understand the Informed Consent Form (ICF), and understand study procedures. 5. The subject has signed the ICF. 6. Available for follow-up for the duration of the study. 7. Agree to abstain from donating blood during and for three months after the end of their participation in the study, or longer if necessary. 8. If heterosexually active female, willing to use an effective method of contraception with partner (oral contraceptive pill; intrauterine device; injectable or implanted contraceptive; condoms incorporating spermicide if using these; physiological or anatomical sterility) from 30 days prior to, and 3 months after, vaccination. Willing to undergo urine pregnancy tests prior to vaccination and blood pregnancy test at screening and final follow up. 9. The subject self-reports at screening that for the past month they have had regular sleep pattern with bedtime occurring between 22:00 and 01:00 h. 10. The subject has venous access sufficient to allow blood sampling as per the protocol. Exclusion Criteria: 1. Significant dietary restrictions (e.g. vegan, lactose intolerant, but vegetarian acceptable) or life-threatening food allergies (e.g. anaphylaxis-related nut allergies). 2. Pregnant or lactating at any point during the study from screening to final follow up. 3. As subjects must be eligible to be randomised to any of the treatment groups they must fulfil the vaccine contraindications eligibility for both group A \& B: 1. Hypersensitivity to the active components of FLUAD, any of the excipients, eggs, chicken proteins, kanamycin and neomycin sulphate, formaldehyde, and cetyltrimetholammonium bromide or those who have had a previous life-threatening reaction to previous influenza vaccinations. 2. Hypersensitivity to the active substances of Agrippal, to any excipients and to residues such as eggs and chicken proteins including ovalbumin. 4. Presence of primary or acquired immunodeficiency states with a total lymphocyte count less than 1,200 per mm3 or presenting other evidence of lack of cellular immune competence e.g. leukaemias, lymphomas, blood dyscrasias, or patients receiving immunosuppressive therapy (including regular use of oral, inhaled, topical or parenteral corticosteroids). 5. Use of any immune suppressing or immunomodulating drugs within 6 months of Visit 1 (screening). 6. Regular use of non-steroidal anti-inflammatory drugs (by any route of administration including topical) within 6 months of Visit 1 (screening) considered by the study physician as likely to interfere with immune responses. 7. Receipt of a vaccine within 30 days of visit 2, or requirement to receive another vaccine within the study period. 8. Presence of an acute severe febrile illness at time of immunisation. 9. History of alcohol, narcotic, benzodiazepine, or other substance abuse or dependence within the 12 months preceding Visit 1. 10. Currently participating in another clinical study with an investigational or non-investigational drug or device, or has participated in a clinical trial within the 3 months preceding Visit 1. 11. Any condition that, in the investigator's opinion, compromises the subject's ability to meet protocol requirements or to complete the study. 12. Receipt of blood products or immunoglobin, or blood donation, within 3 months of screening. 13. Unable to read and speak English to a fluency level adequate for the full comprehension of procedures required in participation and consent. 14. An average weekly alcohol intake that exceeds 14 or 21 units per week for females and males, respectively (1 unit = 12 oz or 360ml of beer; 5oz or 150ml of wine; 1.5oz or 45ml of distilled spirits), or unwilling to stop alcohol consumption for each treatment period during the study. 15. Currently smokes in excess of 5 cigarettes/day or use tobacco or nicotine substitutes (within the last 6 months of screening), or subjects unwilling to refrain from smoking or are unable to abide by Surrey CRC restrictions. 16. Consumes excessive amounts, defined as greater than 4 servings (1 serving is approximately equivalent to 120mg caffeine) of coffee, tea, cola, or other caffeinated beverages/food per day.