Genomic DNA in Human Blastocoele Fluid

Cervesi Hospital, Cattolica, Italy35 enrolled

Overview

The objective will focus on the characterization of DNA fragments present in Blastocoele fluid, and the evaluation of these fragments as potential target for Preimplantation Genetic Diagnosis. To reach this goal, real-time PCR, Whole Genome Amplification techniques and -subsequently- Next Generation Sequencing and aCGH approaches will be used.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Chromosomal Aneuploidies Single Gene Desease

Interventions

blastocoele aspirationOTHER

Eligibility

Sex: FEMALEMin age: 18 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * IVF patient with supernumerary embryos to vitrify at blastocyst stage Exclusion Criteria: * IVF patient without blastocyst formation

Locations (1)

Cervesi Hospital IVF Unit

Cattolica, Rimini, Italy

Outcomes

Primary Outcomes

Number of samples with DNA in blastocoele Fluid

the presence of DNA will be evaluated through rtPCR

Time frame: 6 month

Secondary Outcomes

Number of euploid samples

Array CGH

Time frame: 8 months

Data from ClinicalTrials.gov

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