The purpose of this study is to determine the effects of a single dose of cranberry beverage in healthy adults on the kinetic uptake of polyphenol compounds and polyphenol metabolites, and antioxidative and anti-inflammatory activity.
Participants will be randomized based on age, sex and BMI, and will consume a low-polyphenol diet 2 days prior to the intervention. On the day of the study, baseline blood and urine samples will be collected after an overnight fast. Participants will consume the assigned beverage within 15 minutes and blood collected at 2, 4, 8, and 24 h (10 mL/blood draw; total blood volume per period = 50 mL). Urine will be collected for polyphenol analysis during the following periods: 0-3, 3-6, 6-9, 9-12, 12-24 hours. After a one-week washout period, participants will receive their second and then the third beverage in a cross-over design; blood and urine sampling will be repeated at each of the 3 intervention periods.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
12
Beverage containing cranberry at a dose of 15.2 ounces consumed within 15 minutes
Beverage containing cranberry at a dose of 15.2 ounces consumed within 15 minutes
Beverage absent cranberry at a dose of 15.2 ounces consumed within 15 minutes
WSU Bioactives Research Lab
Pullman, Washington, United States
Reduced Glutathione (GSH) Concentrations in Red Blood Cells
Time frame: 0, 2, 4, 8, 24 h
Superoxide Dismutase (SOD) Activity in Red Blood Cells
Time frame: 0, 2, 4, 8, 24 h
Glutathione Peroxidase (GPx) Activity in Red Blood Cells
Time frame: 0, 2, 4, 8, 24 h
Oxidative Damage to DNA Assessed by Plasma 8-hydroxy-2'-Deoxyguanosine (8-OHdG)
Time frame: 0, 2, 4, 8, 24 h
C-Reactive Protein (CRP) Concentrations in Plamsa
Time frame: 0, 2, 4, 8, 24 h
Nitric Oxide (NO) Concentrations in Plamsa
Time frame: 0, 2, 4, 8, 24 h
Interleukin-1alpha (IL-1a) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
Interleukin-1beta (IL-1b) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
Interleukin-2 (IL-2) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
Interleukin-4 (IL-4) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
Interleukin-6 (IL-6) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
Interleukin-8 (IL-8) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
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Interleukin-10 (IL-10) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
Interferon-gamma (IFN-y) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
Tumor Necrosis Factor-alpha (TNF-a) Concentrations in Plasma
Time frame: 0, 2, 4, 8, 24 h
Urinary Anti-bacteria Adhesion Activity
Time frame: 0, 3, 6, 9, 12, 24 h