Mendelian Reverse Cholesterol Transport Study

Inclusion Criteria: 1. Men and women between the ages of 18 and 75 2. Subjects must be: 1. Carriers of functional mutations of genes encoding proteins affecting HDL metabolism; 2. Healthy control subjects with HDL cholesterol levels within the normal range of the lab where screening tests are run, or at the discretion of the investigator, and matched for gender, race, age (± 5 years) to the patients. 3. Negative screening pregnancy test if female of child bearing potential (females of child-bearing potential must be following a medically accepted form of contraception) 4. Subjects must be able to comprehend and willing to provide a signed IRB approved Informed Consent Form. 5. Subjects must be willing and able to comply with all study-related procedures. Exclusion Criteria: 1. Known cardiovascular disease, including coronary disease, cerebrovascular disease, or peripheral vascular disease (control subjects only) 2. History of diabetes mellitus or fasting glucose \> 126 mg/dL at the screening visit (control subjects only). 3. Any current, unstable endocrine disease as assessed by collection of medical history during screening. Subjects with rare Mendelian disorders with thyroid disease that is well controlled by stable treatment may be considered for enrollment at the discretion of the principal investigator 4. History of previous malignancy, other than basal cell or squamous cell carcinoma of the skin, from which the patient has been disease free for less than 5 years as assessed by collection of medical history during screening 5. Current diagnosis of anemia as assessed by collection of medical history during screening or hemoglobin less than 10 g/dL as evaluated during safety lab at screening 6. History of kidney disease or chronic renal insufficiency, as defined as estimated glomerular filtration rate (eGFR) \< 60 ml/min/1.73m2 in control subjects and patients with other disorders of HDL metabolism and eGFR \< 30 ml/min/1.73m2 in subjects with Lecithin-Cholesterol Acyltransferase (LCAT) deficiency. 7. Any active rheumatologic, pulmonary, or dermatologic disease or inflammatory condition as assessed by collection of medical history during screening, and judged by the investigator to be a major condition. 8. Sustained uncontrolled hypertension (Systolic \>160 mm Hg and/or Diastolic BP \>100 mmHg) at screening. Blood pressure may be re-tested twice after initial assessment in the supine position at five minute intervals (for a total of 3 blood pressure assessments). The pressure elevation is considered sustained if either the systolic or the diastolic pressure values are outside the stated limits for all three assessments 9. Use of warfarin, or any known coagulopathy and /or elevated prothrombin time/partial thromboplastin time (PT/PTT) \>1.5 x upper limit of normal (ULN) 10. Self-reported history of human immunodeficiency virus (HIV) positive 11. History of previous organ transplantation, as assessed by collection of medical history during screening 12. Clinical evidence of liver disease or liver injury as indicated by abnormal liver function tests such as alanine transaminase (ALT) or aspartate transaminase (AST) \> 1.5x ULN, or self-reported history of positive Hepatitis B or Hepatitis C test result 13. Any surgical procedure that occurred within the previous 3 months of the screening visit, as assessed by collection of medical history during screening, and judged by the investigator to be a major procedure. 14. History of drug abuse (\< 1 year), as assessed by collection of medical history during screening procedures 15. Regular abuse of alcoholic beverages (\> 2 drinks/day), as assessed by collection of medical history during screening procedures 16. Self-reported participation in an investigational drug study within 6 weeks prior to the screening visit 17. Serious or unstable medical or psychological conditions that, in the opinion of the investigator, would compromise the subject's safety or successful participation in the study will be excluded. 18. Use of lipid lowering drugs expected to affect RCT (e.g. fibrates) within the 6 weeks prior to dosing or during the study, as assessed by collection of medical history during screening and concomitant medication checks at each study visit. Use of statins (stable dose for at least 30 days) is permitted. 19. Male subjects who plan to conceive a child within 3 months of the conclusion of the study. 20. Women who are pregnant or lactating or who are planning to become pregnant