Fructose-induced Intestinal de Novo Lipogenesis

N/ACompletedNCT01792089

University of Lausanne24 enrolled

Overview

8 healthy volunteers, 8 pre-bypass obese subjects, 8 subjects between 12-18 months post-bypass, and 8 subjects matched to the post-bypass patients will be studied in two sub-studies Sub-study 1) after ingestion of two or three different of the following test-meals: A)cream and whey protein B)cream, whey protein and fructose + 13C6 fructose C)test meal with cream, whey protein, fructose and glucose + 13C6 fructose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood Sub-study 2) after ingestion of two or three different of the following test-meals: A)cream labelled with 13C6 palmitic acid and whey protein B))cream labelled with 13C6 palmitic acid, whey protein, fructose and glucose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood in sub-study 1 Their exogenous lipid absorption kinetics will be assessed by measuring 13C palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in sub-study 2

Study Type

OBSERVATIONAL

Enrollment

Conditions

Obesity

Interventions

fat+protein mealOTHER

Eligibility

Sex: ALLMin age: 25 YearsMax age: 50 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * age 18-50 years * sex male or female * less than 2 kg body weight change over the past 3 months subjects Exclusion Criteria: * antidiabetic and hypolipemic drugs * alcohol consumption \>20g/day * severe eating disorders * severe psychological problems * vegetarian diet or other specific diet * consumption of illicit substances * pregnancy * weight gain or weight loss \> 1 kg in the last 3 months

Outcomes

Primary Outcomes

Intestinal de novo lipogenesis (sub-study 1)

Measurement of total TG and 13C palmitate in chylomicrons: The sf \>20 fraction of post-prandial blood samples will be prepared, and non-chylomicrons, apoB100-containing lipoprotein will be removes by immuno-affinity chromatography using a specific anti-apoB100 antibody

Time frame: 0-8-hour post-prandial

Postprandial exogenous, labelled palmitate concentration (sub-study 2)

Measurement of total TG and 13C palmitate in chylomicrons of sf \>20 fraction of post-prandial blood samples will be prepared,

Time frame: 0-8-hour post-prandial

Secondary Outcomes

post-prandial triglyceride profile (sub-studies 1 and 2

Triglyceride will be measured in the chylomicron, VLDL, LDL and HDL fractions of post-prandial blood samples

Time frame: 0-8 hour post-prandial