Bacteriophage Effects on Pseudomonas Aeruginosa

University Hospital, Montpellier59 enrolled

Overview

Pulmonary phage therapy to treat bacterial infections of the respiratory tract have been investigated in animals. The aim of the present study is to evaluate the efficacy of bacteriophages in infecting Pseudomonas aeruginosa (PA) strains present in sputum samples. A cocktail of 10 bacteriophages will be applied on 60 sputum samples obtained from cystic fibrosis (CF) patients during 6 hours.We will determine the bacteria and bacteriophages strains in sputum samples collected. Then the sensitivity of individual colony will be tested.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Cystic Fibrosis

Interventions

Collection of induced sputum in order to evaluate the efficacy of a cocktail of 10 bacteriophages.OTHER

Collection of induced sputum on cystic fibrosis patients in order to evaluate the efficacy of a cocktail of 10 bacteriophages.

Eligibility

Sex: ALLMin age: 6 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Informed consent * Affiliated or benefit from a disease insurance regimen * Men and women * Aged from at least 6 years old * Confirmed Diagnosis of cystic fibrosis based on presence of 2 mutations of CFTR gene and/or 2 positive tests of sweat chloride and/or 2 measures of pathologic difference of nasal potential associated to cystic fibrosis clinical signs. * Patients able to produce sputum * Pseudomonas aeruginosa Chronic infected patients Exclusion Criteria: * Simultaneous participation to another project on anti-infection, anti-inflammatory or modificating agents * Subjet in exclusion period * Law protected patient * Realisation of sputum production is contra-indicated

Locations (1)

CHU de Montpellier - Hôpital Arnaud de Villeneuve CRCM

Montpellier, Languedoc Roussillon, France

Outcomes

Primary Outcomes

Pseudomonas aeruginosa (PA) strains counts after 6 hours in presence of bacteriophage

Time frame: after 6 hours in presence of bacteriophage

Secondary Outcomes

Pseudomonas aeruginosa (PA) strains counts after 24 hours in presence of bacteriophage

Time frame: after 24 hours in presence of bacteriophage

Bacteriophage counts after 6 hours incubation within sputum samples

Time frame: after 6 hours incubation within sputum samples

Sensitivity of individual Pseudomonas aeruginosa (PA) colonies to bacteriophages

Time frame: Sensitivity of individual PA colonies to bacteriophages

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.