Testing Pfs25-EPA/Alhydrogel as a Potential Malaria Transmission Blocking Vaccine

* INCLUSION CRITERIA: All of the following criteria must be fulfilled for a subject to participate in this trial: 1. Adult age 18 45 years. 2. Known residents of the village of Bancoumana or immediate surrounding areas. 3. Available for the duration of the trial (approximately 2.5 years). 4. Good general health as a result of review of medical history and/or clinical testing at the time of screening. 5. Willingness to participate in the study as evidenced by signing the informed consent document, or by fingerprinting the consent document with the signature of a witness. 6. Willingness to undergo a HIV test. 7. Willingness to undergo direct skin feeds. EXCLUSION CRITERIA: A subject will be excluded from participating in this trial if any one of the following criteria is fulfilled: 1. Pregnancy, as determined by a positive urine or serum human choriogonadotropin (beta human chorionic gonadotropin ) test at any point during the study (if female). 2. Currently lactating and breast-feeding (if female). 3. Unable or unwilling to use reliable contraception for a minimum of one month prior to the first vaccination to three months after the last vaccination (if female). Reliable birth control includes: pharmacologic contraceptives including oral, parenteral, and transcutaneous delivery; condoms with spermicide; diaphragm with spermicide; surgical sterilization; vaginal ring; transdermal patch; intrauterine device; abstinence; and postmenopause. (Note: If screening of the female subject occurs \< 1 month prior to first vaccination, a negative serum pregnancy test at time of screening and at enrollment (first vaccination) and agreement to use of reliable contraception for the duration of the study until three months after the fourth vaccination is acceptable.) 4. Behavioral, cognitive, or psychiatric disease that in the opinion of the investigator affects the ability of the participant to understand and cooperate with the study protocol. 5. Hemoglobin, WBC, and platelets outside the local laboratory-defined upper limit of normal (subjects may be included at the investigator s discretion for not clinically significant values outside of normal range). 6. Neutropenia (absolute neutrophil count \<1250/mm3). 7. Alanine transaminase (ALT) or creatinine (Cr) level above the local laboratory-defined upper limit of normal. 8. Positive test for hepatitis C virus (HCV). 9. Positive test for hepatitis B (HBsAg). 10. Positive test for human immunodeficiency virus (HIV). 11. Known immunodeficiency syndrome. 12. Evidence of clinically significant neurologic, cardiac, pulmonary, hepatic, endocrine, rheumatologic, autoimmune, hematological, or renal disease by history, physical examination, and/or laboratory studies including urinalysis. 13. Subject has had medical, occupational, or family problems as a result of alcohol or illicit drug use during the past 12 months. 14. History of a severe allergic reaction or anaphylaxis. 15. History of a severe reaction to mosquito bites. 16. Severe asthma, defined as asthma that is unstable or required emergent care, urgent care, hospitalization or intubation during the past 2 years, or that has required the use of oral or parenteral corticosteroids at any time during the past 2 years. 17. Clinically significant reactive airway disease that does not respond to bronchodilators. 18. History of a surgical splenectomy. 19. Use of chronic (greater than or equal to 14 days) oral or intravenous corticosteroids (excluding topical or nasal) at immunosuppressive doses (i.e., prednisone \>10 mg/ day) or immunosuppressive drugs within 30 days of starting this study. 20. Receipt of a live vaccine within past four weeks or a killed vaccine within past two weeks prior to entry into the study. 21. Receipt of blood products within the past 6 months. 22. Previous participation in a malaria vaccine trial. 23. History of receiving any investigational product within the past 30 days. 24. Refusal to allow storage of samples for future research at the time of enrollment. 25. Any medical, psychiatric, social, or occupational condition that, in the judgment of the Principal Investigator (PI), would interfere with the evaluation of study objectives or increase risk to the subject.