Optimization of Time-of-Flight Mass Cytometry (CyTOF) Analysis for Evaluation of Immune Changes Following Surgery

Martin Angst5 enrolled

Overview

Surgical trauma triggers a massive inflammatory response. Over time, both the innate and adaptive branches of the immune system are affected by surgical trauma. The purpose of this study is to use a single cell flow cytometry approach to characterize the cellular and molecular mechanisms involved in the inflammatory response to surgical trauma.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Surgical Trauma

Eligibility

Sex: ALLMin age: 18 YearsMax age: 90 Years

Medical Language ↔ Plain English

Inclusion Criteria: 1. Ages 18 - 90 2. Men and women 3. Undergo back surgery Exclusion Criteria: 1. Any systemic disease that might compromise the immune system (e.g. lupus) 2. Current diagnosis of cancer 3. Any condition that, in the opinion of the investigator, might compromise the integrity of the study or safety of the participant. 4. Unwilling to sign informed consent

Locations (1)

Stanford University Hospital

Stanford, California, United States

Outcomes

Primary Outcomes

Numerical (cell frequencies) and functional (phosphorylation of signaling proteins and transcription factors) changes of all circulating immune cells

This is an exploratory study.

Time frame: Immediately before to surgery, 1 hour after surgery, 24 hours after surgery, and day of hospital discharge

Data from ClinicalTrials.gov

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