To investigate gene expression profile and immunological associated analysis relating to immunotherapy response of patients with malignant tumor presenting malignant cavity effusion after DC-CIK immunotherapy.
1. The patients with malignant cavity effusion are treated with dendritic cells (DC) plus cytokine induced killer cells (CIK) locally. 2. Malignant cavity effusion from cancer patients is obtained through puncture and is centrifugalized to get supernatant fluid and enrich cancer cells before and after the therapy. 3. The enriched cancer cells which are flash frozen, as well as the supernatant, are stored at -80°C until processing. 4. The T-Cell Receptor/B-Cell Receptor gene expression in cavity effusion is detected by micro-array to explore the mechanism that DC-CIK immunotherapy controls the malignant cavity effusion. 5. Statistical analysis is performed using unsupervised hierarchical cluster.
Study Type
OBSERVATIONAL
Enrollment
30
The patients with malignant cavity effusion are treated with dendritic cells (DC) plus cytokine induced killer cells (CIK) locally.
Capital Medical University Cancer Center/Beijing Shijitan Hospital
Beijing, Beijing Municipality, China
Immunotherapy response
T-Cell Receptor and B-Cell Receptor gene expression profiling and the change of cytokines, lymphocytes subpopulation before and after DC-CIK infusion
Time frame: 1 month
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