Storage Temperature and Quality of Leucoreduced Whole Blood

Haukeland University Hospital87 enrolled

Overview

In a combat situation the need for safe and high-quality blood products is increasing. WB (Whole Blood) is used in many trauma situations, but we wish to investigate whether there is an even better whole blood product. The purpose of this study is to examine the quality of leucoreduced, cold-stored whole blood. We also wish to examine the effects of filtration of whole blood without a 1-2 hour holding-time (warm-filtration) and the effects of forced filtration. We also wish to explore the possibility of using cold-stored WB for production of RCC. Analyses are performed with a main focus to investigate function and quality of red blood cells and platelets.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

TREATMENT

Masking

SINGLE

Enrollment

Conditions

Trauma Austere Environment Blood Bank Buddy Transfusion

Eligibility

Sex: ALLMin age: 18 YearsMax age: 70 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy volunteer * Age between 18 and 70 * Negative pregnancy test (women in fertile age) * Blood pressure range: (160-100)/(100-40) * Pulse range: 40-100 Exclusion Criteria: * Usage of platelet-influencing medication * Mild flu or more severe illnesses * Open wound * Pregnancy or trying to get pregnant

Outcomes

Primary Outcomes

Platelet function

Platelet function is assessed by various parameters, such as thrombelastography (TEG), Multiplate and flow cytometry (Lactadherin, Calcein violet AM, ++).

Time frame: After 10 days of cold-storage

Secondary Outcomes

Leukocyte count

Filtered whole blood should have a concentration less than 1\*10\^6 cells/L to avoid transfusion reactions.

Time frame: After leukocyte filtration