The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
37
Interval training 3 times a week for 8 weeks
Moderate training 3 times a week for 8 weeks
Institute for Circulation and Medical Imaging, NTNU
Trondheim, Norway
DNA-methylation
From the skeletal muscle biopsies RNA and DNA was isolated, DNA was further bi-sulfite converted and DNA-methylation was analyzed using the Illumina HumanMethylation450 BeadChip. The RNA was further used for gene expression analyses by real-time RTqPCR.
Time frame: 8 weeks
Gene Expression
Time frame: 8 weeks
Maximal Aerobic Capacity
measured on treadmill at baseline and after eight weeks, also biopsies from Vastus lateralis were sampled.
Time frame: 8 weeks
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