This study is intended to evaluate ovarian cells after being submitted do different in vitro fertilization protocols. The investigators will analyze hormones secretion and intracellular mechanisms of these cells comparing the GnRH agonist protocol and the GnRH antagonist protocol.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
28
Projeto Cegonha
Porto Alegre, Rio Grande do Sul, Brazil
RECRUITINGInsemine
Porto Alegre, Rio Grande do Sul, Brazil
RECRUITINGEvaluation of steroid secretion and enzyme expression in in vitro luteinized granulosa cells
evaluation of granulosa cells hormone secretion and enzyme expression in media culture 48 hours after ovarian punction
Time frame: after 48h in media culture
Number of retrieved oocytes
Immediately after the ovarian punction the number of retrieved oocytes will be evaluated
Time frame: After the ovarian punction
number of embryos
the embryologist will verify the number of embryos after 3 to 5 days from the ovarian punction
Time frame: 3 to 5 days after ovarian punction
Subgroup analyses comparing FSH versus HMG protocols
It will be verified the differences between the two types of gonadotrophins in ovarian stimulation regarding: units of gonadotrophins used, number of retrieved oocytes,number of embryos and embryo score
Time frame: after ovarian punction
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