Endothelial Function in Patients With Type 2 Diabetes Versus Healthy Subjects

Patients with T2D: Inclusion criteria - 1. Men and women aged 30-65 years 2. Body Mass Index in the range of 25 - 45(kg/m2) 3. Type 2 diabetes mellitus for at least one year 4. Treatment of T2D: diet or oral antidiabetic agents (stable drug therapy at least 3 months before the trial 5. The presence of metabolic syndrome - any three of the following symptoms: 1. Abdominal obesity - waist circumference in men \>102 cm, in women \>88 cm 2. Diagnosis and treatment of type 2 diabetes 3. Raised blood pressure (BP): systolic BP \> 130 mm Hg or diastolic BP \>85 mm Hg, or treatment of previously diagnosed hypertension 4. Reduced HDL cholesterol in men \< 1 mmol/l, in women \< 1,3 mmol/l (or treatment) 5. Raised triglycerides \> 1,7 mmol/l (or treatment) 4\. HbA1c ≥ 42 a ≤100 mmol/mol Exclusion criteria - 1. Type 1 diabetes mellitus 2. Unstable drug therapy at least 3 month before the trial 3. Pregnancy, breast feeding or trying to become pregnant 4. Alcoholism or drug use 5. Presence of other medical condition, which occurs during physical examination, laboratory tests, including pulmonary, neurological or inflammatory disease, which would be considered by the examiner to distort the consistency of data Healthy subjects: Inclusion criteria - 1. Men and women aged 30-65 years 2. Body Mass Index 25-45 (kg/m2) 3. Absence of metabolic syndrome - not more than any two of the following symptoms: 1. Abdominal obesity - waist circumference in men \>102 cm, in women \>88 cm 2. Diagnosis and treatment of type 2 diabetes or raised fasting plasma glucose level (FPG \>5,6 mmol/l) 3. Raised blood pressure (BP): systolic BP \>130 mm Hg or diastolic BP \>85 mm Hg, or treatment of previously diagnosed hypertension 4. Reduced HDL cholesterol in men \<1 mmol/l, in women \<1,3 mmol/l(or treatment) 5. Raised triglycerides \> 1,7 mmol/l (or treatment) Exclusion criteria - 1. Diabetes or impaired glucose tolerance (HbA1c ≥40 mmol/mol and/or FPG \>5,6 mmol/l), diagnosed diabetes in first-line relatives 2. Pregnancy, breast feeding or trying to become pregnant 3. Alcoholism or drug use 4. Presence of other medical condition, which occurs during physical examination, laboratory tests, including pulmonary, neurological or inflammatory disease, which would be considered by the examiner to distort the consistency of data