Nanosilver Fluoride to Prevent Dental Biofilms Growth

Phase 1CompletedNCT01950546

University of Pernambuco30 enrolled

Overview

This study aims to evaluate the effectiveness of nanosilver fluoride for controlling the growth of S. mutans present in dental plaque of children.

The product will be apllied on cervical vestibular surfaces of incisors and canines healthy, with a total of 8 teeth, 4 upper and 4 below. Children will be instructed not to ingest any liquid or food within two hours after application. After a period of 7 days will be carried biofilm colect of cervical dental surfaces of incisors and / or canines upper and lower, with the aid of sterile spatulas. This material will be packaged in pre-weighed microfuge tubes containing 1.5 ml of buffered saline solution (0.9% sodium chloride), and transported under refrigeration immediately to the laboratory where analysis will be performed and the S. mutans colonies will be counted. The biofilm collect and posterior laboratorial analysis will be performed weekly.

Study Type

INTERVENTIONAL

Allocation

Purpose

TREATMENT

Masking

DOUBLE

Enrollment

Conditions

Dental Caries

Interventions

nanosilver fluorideDRUG

The product will be applied in the volunteers in the morning, before the first class, in relative isolation, made with cotton rolls and Vaseline to protect the soft tissue. It will apply 30mg of the product with microbrush, previously standardized in a pilot study on cervical vestibular surfaces of incisors and canines healthy, on a total of 8 teeth, 4 upper and 4 lower. After a period of 7 days,the cervical dental biofilms will be collect, third on the buccal surfaces of incisors and / or canines upper and lower, with the aid of sterile spatulas.

Eligibility

Sex: ALLMin age: 5 YearsMax age: 6 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Have at least 4 upper teeth and 4 lower teeth deciduous;systemic health satisfactory; did not use antibiotics or nonsteroidal anti-inflammatory within one month before or during the study;do not use any other forms of chemical control of biofilm during the study period;not being a user of orthodontic devices or dentures; Exclusion Criteria: * Presenting oral lesions, supragingival calculus and severe malocclusion.

Locations (1)

Escola Municipal Anita Trigueiro do Valle

João Pessoa, Paraíba, Brazil

Outcomes

Primary Outcomes

Initial biofilm collecting before applying the product and after nanosilver fluoride application.

The initial dental biofilm collection will be conducted to count colony-forming units of S. mutans and then the product will be applied on these dental surfaces after the collect. After one week, a new collect will be performed and the colony-forming units of S. mutans will be counting to compare with the initial.

Time frame: one week

Secondary Outcomes

Evaluation of effectiveness of nanosilver fluoride on bacterial growth in the dental biofilm

Collection of dental biofilm will be performed after two weeks of product application and colony count will be performed in the laboratory.

Time frame: two weeks

Data from ClinicalTrials.gov

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