The main objective of this study is to compare the intestinal microbiota in newly diagnosed rheumatoid arthritis (RA) with that from individuals without RA. The first analysis will compare the proportion of filamentous bacteria in the intestinal microbiota between the two groups.
The secondary objectives of this study are : * A. To compare between the two groups : * a. The proportion of Proteus mirabilis in the intestinal microbiota; * b . The proportion of species producing peptidyl arginine deaminase (PAD ) in the intestinal microbiota; * c . The number of species found in the intestinal microbiota. * B. To evaluate bacterial translocation (from the digestive tract) in newly diagnosed RA patients with and healthy volunteers without RA. (Bacterial translocation is a phenomenon in which live bacteria or a part of bacteria (e.g. LPS teichoïc acid) cross the intestinal barrier and reach the bloodstream.). * C. To describe the ecology of the intestinal microbiota of newly diagnosed RA patients (characterization of species found according to their respiratory metabolism, according to their bacteriological characteristics, and according to whether or not the species are found significantly more frequently in patients with RA than in individuals without RA).
Study Type
OBSERVATIONAL
Enrollment
55
1 ml of plasma will be obtained (blood sample). DNA will be extracted and amplified, and real-time quantitative PCR (polymerase chain reaction) performed.
DNA from stool samples will be extracted, amplified, and used to identify the bacteria species present.
CHRU de Montpellier - Hôpital Lapeyronie
Montpellier, France
CHRU de Nîmes - Hôpital Universitaire Carémeau
Nîmes, France
Unité U1047 "Bacterial virulence and infectious diseases", UFR de Médecine
Nîmes, France
The proportion of filamentous bacteria in the intestinal microbiota
Time frame: Baseline (day 0)
The proportion of Proteus mirabilis in the intestinal microbiota
Time frame: baseline (Day 0)
The proportion of species producing peptidyl arginine desaminase (PAD) in the intestinal microbiota
Time frame: baseline (Day 0)
The number of species found in the intestinal microbiota
Time frame: baseline (Day 0)
Presence/absence of bacterial translocation
Time frame: baseline (Day 0)
Description of the ecology of intestinal microbiota
Diversity indices according to species and/or functional groups. The general goal is to provide a description of the functional differences between groups.
Time frame: baseline (Day 0)
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