Effects of Oxygen Status on Endotoxemia Induced Inflammation and Hypoxia Inducible Factor-1α

Inclusion Criteria: * Written informed consent to participate in this trial * Male subjects aged 18 to 35 years inclusive * Healthy as determined by medical history, physical examination, vital signs, 12-lead electrocardiogram, and clinical laboratory parameters Exclusion Criteria: * Use of any medication(including herbal remedies and vitamin/mineral supplements) or recreational drugs within 7 days prior to profiling day * Smoking * Use of caffeine, or alcohol or within 1 day prior to profiling day * Previous participation in a trial where LPS was administered * Surgery or trauma with significant blood loss or blood donation within 3 months prior to profiling day * Participation in another clinical trial within 3 months prior to profiling day. * History, signs or symptoms of cardiovascular disease * An implant that in the opinion of the investigator may make invasive procedures risky for the subject due to the increased risks associated with a possible infection. * Subject has an implanted active cardiac device (ICD, IPG and/or CRT) Implanted active neurostimulation device * Subject has internal jugular vein that cannot be accessed * History of vaso-vagal collapse or of orthostatic hypotension * History of atrial or ventricular arrhythmia * Resting pulse rate ≤45 or ≥100 beats / min * Hypertension (RR systolic \>160 or RR diastolic \>90) * Hypotension (RR systolic \<100 or RR diastolic \<50) * Conduction abnormalities on the ECG consisting of a 1st degree atrioventricular block or a complex bundle branch block * Subject is diagnosed with epilepsy or history of seizures * Renal impairment: plasma creatinine \>120 μmol/L * Liver function abnormality: alkaline phosphatase\>230 U/L and/or ALT\>90 U/L * Coagulation abnormalities: APTT or PT \> 1.5 times the reference range * History of asthma * Immuno-deficiency CRP \> 20 mg/L, WBC \> 12x109/L, or clinically significant acute illness, including infections, within 2 weeks before profiling day * Known or suspected of not being able to comply with the trial protocol - Inability to personally provide written informed consent (e.g. for linguistic or mental reasons) and/or take part in the study.