Irisin, a newly discovered myokine induced in exercise, has potential effects in stimulating adipose tissue browning, fighting obesity and diabetes. No prior study has reported on the role of circulating irisin in healthy individuals in correlation with lean and fat body mass. Furthermore, the circadian and seasonal variation of irisin is largely unknown.
The main aims of this prospective study will be to investigate: 1) circulating irisin levels in healthy, young men and women according to their lean and fat mass; 2) the circadian variations of irisin levels; 3) the seasonal variations of irisin levels.
Study Type
OBSERVATIONAL
Enrollment
122
After an overnight fast, blood samples will be obtained before as well as 30 min after standardized mixed meal ingestion
After an overnight fast and rest, blood samples will be obtained before as well as 30 min after aerobic exercise
424 General Military Hospital
Thessaloniki, Greece
Irisin
After a standardized dinner and an overnight fast, a blood sample will be obtained the next morning.
Time frame: 1 day
irisin circadian variation
20 participants will be hospitalized and closely monitored for 24 hours. A catheter will be inserted in a vein and blood samples will be obtained every 3 hours through the 24-hour period
Time frame: 1 day
irisin seasonal variation
20 participants will be monitored for one year and blood samples will be obtained every 3 months (at the middle of month January - April - July - October). At the same intervals physical examination and BMI calculation will be conducted. Subjects will be instructed to maintain their normal exercise routine and dietary habits.
Time frame: 12 months
Irisin postprandial
A sub-population of 40 participants of the cohort will receive a standardized meal (Boost) in two different quantities (20 participants will receive 125 ml and 20 participants 250 ml). Serum irisin levels will be measured at baseline and 30 min postprandial.
Time frame: 1 day
Irisin after excercise
A sub-population of 20 participants of the cohort will be subjected to 30 min of aerobic exercise. Serum irisin levels will be measured at baseline and at 30 min.
Time frame: 1 day
Irisin associates
After a standardized dinner and an overnight fast, a blood sample for adiponectin and leptin will be obtained the next morning, and body composition (total body fat mass and bone-free lean mass) will be assessed by BIA. Participants will also be asked to complete a validated questionnaire assessing their diet and exercise habits. Correlations between serum irisin levels and the above parameters will be examined
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Time frame: 1 day
serum adipokines
After a standardized dinner and an overnight fast, a blood sample for adiponectin and leptin will be obtained the next morning.
Time frame: 1 day