The purpose of this study is to determine whether oral vitamin D supplementation improves the clinical severity of atopic dermatitis in children. In addition, this study plans to evaluate the effects of vitamin D supplementation on several key aspects of the immune system of children with atopic dermatitis.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
QUADRUPLE
Enrollment
101
School of Medicine, Pontificia Universidad Católica de Chile
Santiago, Chile
Change in SCORAD index
Change in Scoring Atopic Dermatitis (SCORAD) index after 6 weeks of vitamin D3 (VD3) supplementation or placebo in children with atopic dermatitis.
Time frame: baseline and 6 weeks
Changes in Th2 immunity
Eosinophil blood counts, serum IgE, Th2 lymphocytes among stimulated PBMCs, serum CCL17, CCL22, and CCL27.
Time frame: baseline and 6 weeks
Change in dendritic cell-mediated tolerance and regulatory T cells
Number and phenotype of blood dendritic cells and number of regulatory T cells.
Time frame: baseline and 6 weeks
Effect of VD3 supplementation on immunity to Staphylococcus aureus
Serum cathelicidin levels, S. aureus skin carriage, and specific IgE to staphylococcal enterotoxins.
Time frame: baseline and 6 weeks
Vitamin D receptor single nucleotide polymorphisms
Effect of VDR SNPs on the VD3 response.
Time frame: baseline and 6 weeks
Change in epidermal protein expression
Gene expression of epidermal proteins by PCR obtained from lesional and non-lesional tape stripping samples.
Time frame: 6 weeks
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