Identification of Predictive Biomarker of Regorafenib in Refractory Colorectal Cancer

Inclusion Criteria: 1. Signed informed consent obtained before any study-specific procedures. 2. Age ≥ 20 3. Pathologically confirmed metastatic adenocarcinoma of colon or rectum 4. Failure of standard therapies, which must include fluoropyrimidine, oxaliplatin, and irinotecan. Failure is defined as progression during or within 3 months following the last administration of therapy. Patients who have withdrawn from standard treatment due to unacceptable toxicity warranting discontinuation of treatment and precluding retreatment with the same agent before progression of disease will also be allowed into the study. Patients treated with oxaliplatin in an adjuvant setting who have progressed during or within 6 months of completion of adjuvant therapy are regarded as failure of oxaliplatin. Patients may or may not have received bevacizumab or cetuximab. 5. Measurable or nonmeasurable disease according to RECIST criteria, version 1.1. 6. Adequate tissue for gene sequencing (surgical FFPE specimen or fresh-frozen biopsy specimen) 7. ECOG PS 0 or 1 8. Life expectancy of at least 3 months 9. Adequate bone-marrow, liver, and renal function as assessed by the following laboratory requirements conducted within 14 days of starting to study treatment * Total bilirubin ≤1.5 × ULN * Alanine aminotransferase and aspartate aminotransferase ≤2 × ULN (≤5 × ULN for patients with liver involvement of cancer) * Amylase and lipase ≤1.5 × ULN * Serum creatinine ≤1.5 × ULN * Glomerular filtration rate ≥30 ml/min/1.73 m2 according to the Modified Diet in Renal Disease abbreviated formula * International normalised ratio (INR) and partial thromboplastin time (PTT) ≤1.5 × ULN. Subjects who are therapeutically treated with an agent such as warfarin or heparin will be allowed to participate provided that no prior evidence of an underlying abnormality in coagulation parameters exists. * Platelet count ≥100,000/mm3, haemoglobin \>9 g/dl, absolute neutrophil count \>1,500/mm3 * Alkaline phosphatase limit ≤2.5 × ULN (≤5 × ULN for patients with liver involvement of their cancer) Exclusion Criteria: 1. Prior treatment with regorafenib 2. Major surgical procedure, open biopsy, or significant traumatic injury within 28 days before start of study medication 3. Pregnancy or breast-feeding. Women of childbearing potential must have a negative pregnancy test performed a maximum of 7 days before start of treatment 4. Congestive heart failure of NYHA class 2 or worse 5. Unstable angina, new-onset angina (begun within the last 3 months). Myocardial infarction less than 6 months before start of study drug 6. Cardiac arrhythmias requiring anti-arrhythmic therapy (beta blockers or digoxin are permitted) 7. Uncontrolled hypertension (systolic blood pressure \>150 mmHg or diastolic \>90 mmHg despite optimal medical management) 8. Arterial or venous thrombotic or embolic events within the 6 months before start of study medication 9. Ongoing infection higher than NCI-CTCAE v4.0 grade 2 10. Known history of HIV infection 11. Active hepatitis B or C virus infection 12. Seizure disorder requiring medication 13. Symptomatic metastatic brain or meningeal tumors 14. History of organ allograft 15. Non-healing wound, ulcer, or bone fracture 16. Interstitial lung disease with ongoing signs and symptoms at the time of informed consent 17. Persistent proteinuria of NCI-CTCAE v4.0 grade 3 or higher 18. Inability to swallow oral medications 19. Any malabsorption condition 20. Unresolved toxicity higher than NCI-CTCAE v4.0 grade 1 attributed to any prior therapy/procedure, excluding alopecia and oxaliplatin-induced neurotoxicity of grade 2 or less