Development of Endothelial Biomarkers

Inclusion Criteria: * \- 40 to 65 years old * male * non-smokers Specific inclusion criteria for healthy subjects : * Healthy subjects should not take antihypertensive or statin. * Healthy subjects should not present metabolic syndrome but one criterion of the metabolic syndrome will be tolerated. Specific inclusion criteria for subjects with metabolic syndrome : \- subjects with metabolic syndrome must have at least 3 of the 5 criteria associated with the metabolic syndrome. Exclusion Criteria: * treatment vasodilator nitric oxide liberating, * diabetes and coronary artery disease, * chronic alcoholism, * severe hepatic impairment, * end stage renal failure or dialysis, * neurological tremor, * cancer, mental illness or other severe disease which can impact informed consent and / or results, * Refusal to be registered in the National File of Volunteers * Person in exclusion of the National Volunteer File